Checkerboard analysis of neutrophil locomotion in response to FMLP
. | FMLP below the filter, nM . | . | . | ||
|---|---|---|---|---|---|
| FMLP, above the filter, nM . | 0 . | 1 . | 10 . | ||
| Without RBC supernatant | |||||
| 0 | 51.5 ± 7.3 | 102.4 ± 9.8 (55.5 ± 5.5) | 112.6 ± 8.8 (61.2 ± 6.2) | ||
| 1 | — | 64.1 ± 2.9 | 91.3 ± 12.7 (70.2 ± 2.2) | ||
| 10 | — | — | 79.3 ± 3.9 | ||
| With RBC supernatant | |||||
| 0 | 40.5 ± 2.0 | 60.7 ± 3.2 (41.4 ± 2.0) | 66.5 ± 7.6 (43.5 ± 3.1) | ||
| 1 | — | 44.2 ± 2.2 | 55.8 ± 6.9 (46.4 ± 2.9) | ||
| 10 | — | — | 52.1 ± 5.5 | ||
. | FMLP below the filter, nM . | . | . | ||
|---|---|---|---|---|---|
| FMLP, above the filter, nM . | 0 . | 1 . | 10 . | ||
| Without RBC supernatant | |||||
| 0 | 51.5 ± 7.3 | 102.4 ± 9.8 (55.5 ± 5.5) | 112.6 ± 8.8 (61.2 ± 6.2) | ||
| 1 | — | 64.1 ± 2.9 | 91.3 ± 12.7 (70.2 ± 2.2) | ||
| 10 | — | — | 79.3 ± 3.9 | ||
| With RBC supernatant | |||||
| 0 | 40.5 ± 2.0 | 60.7 ± 3.2 (41.4 ± 2.0) | 66.5 ± 7.6 (43.5 ± 3.1) | ||
| 1 | — | 44.2 ± 2.2 | 55.8 ± 6.9 (46.4 ± 2.9) | ||
| 10 | — | — | 52.1 ± 5.5 | ||
Results are expressed as the distance migrated by the leading front of cells, μm/45 min. Numbers in parentheses show the calculated distance migrated if cells would have not responded to the gradient of FMLP, but would have moved randomly in the gradient. Calculations were performed according to Zigmond and Hirsch.24 Data are means ± 1 SD; n = 3. — indicates not done