Table 4.

Real-time PCR analysis of hTERT expression upon activation in freshly isolated naive and memory T cells


Donor

T-cell population

Nonstimulated

Stimulated

Fold increase in hTERT expression*

hTERT expression relative to naive cells
1   Naive   0.075  8.474   113.3   1  
1   Memory   0.042   2.051   49.0   0.43  
2   Naive   0.027   3.289   120.0   1  
2   Memory   0.011   0.889   78.3   0.65  
3   Naive   0.019   1.014   52.4   1  
3
 
Memory
 
0.015
 
0.616
 
41.2
 
0.79
 

Donor

T-cell population

Nonstimulated

Stimulated

Fold increase in hTERT expression*

hTERT expression relative to naive cells
1   Naive   0.075  8.474   113.3   1  
1   Memory   0.042   2.051   49.0   0.43  
2   Naive   0.027   3.289   120.0   1  
2   Memory   0.011   0.889   78.3   0.65  
3   Naive   0.019   1.014   52.4   1  
3
 
Memory
 
0.015
 
0.616
 
41.2
 
0.79
 
*

Increase in hTERT expression upon activation

Up-regulation of hTERT expression relative to naive cells

Values are corrected for the expression of the housekeeping gene human TBP in nonstimulated cells of each population

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