Table 6.

Fas-expressing LTC-ICs are not compromised by Fas activation

Fold expansion
LTC-CFCs per 50 or 75 CD34+ CD38− cells, (replicate values)
Without CH11
With CH11
Without CH11 With CH11
Experiment
Without TNF
With TNF
Without TNF
With TNF
Without TNF*
 Without TNF†
Exp 1, CB   23   21   18   7   205(120-195-300)   287(195-215-450)  
Exp 2, CB   160   100   180   90   50(37-41-52-69)   40(35-37-39-48)  
Exp 3, CB   331   109   309   100   290 (75-285-510)   350 (270-360-420)  
Exp 4, BM   52   56   58   60   42 (35-37-48-48)   43 (35-37-47-52)  
Exp 5, BM   68   42   52   40   43 (22-37-37-41-52-71)   31 (19-29-34-37-37)  
Exp 6, BM
 		 70
 		 50
 		 46
 		 48
 		 42 (34-37-45-48-48)
 		 40 (32-35-37-48-50)
 
Fold expansion
LTC-CFCs per 50 or 75 CD34+ CD38− cells, (replicate values)
Without CH11
With CH11
Without CH11 With CH11
Experiment
Without TNF
With TNF
Without TNF
With TNF
Without TNF*
 Without TNF†
Exp 1, CB   23   21   18   7   205(120-195-300)   287(195-215-450)  
Exp 2, CB   160   100   180   90   50(37-41-52-69)   40(35-37-39-48)  
Exp 3, CB   331   109   309   100   290 (75-285-510)   350 (270-360-420)  
Exp 4, BM   52   56   58   60   42 (35-37-48-48)   43 (35-37-47-52)  
Exp 5, BM   68   42   52   40   43 (22-37-37-41-52-71)   31 (19-29-34-37-37)  
Exp 6, BM
 		 70
 		 50
 		 46
 		 48
 		 42 (34-37-45-48-48)
 		 40 (32-35-37-48-50)
 

The expansion equivalent (EE) of 50 CB or 75 BM CD34+CD38− cells were cultured for 5 (experiments 1,2, 4-6) or 7 days (experiment 3) in SF medium supplemented with SFT3 in the absence or presence of TNF (2 to 20 ng/mL) and/or CH11 (1 μg/mL) as indicated. Cultures were subsequently evaluated for 6-week LTC-CFC activity (“Materials and methods”). Mean values are presented with replicate values in parentheses.

Abbreviations are explained in Table 3.

*

Data for all cultures without CH11 but with TNF were 0 (0-0-0).

†

For cultures with both CH11 and TNF, data were 0 (0-0-0) for experiments 1 and 3, and 0 (0-0-0-0) for the other experiments.

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