Effects of 17-AAG on the cell-cycle status of HL-60/Neo and HL-60/Bcl-2 cells
. | % of cells in designated cell phase at various hours . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell phase . | 0 . | 2 . | 4 . | 6 . | 8 . | 24 . | 32 . | 48 . | |||||||
| HL-60/Neo | |||||||||||||||
| G0/G1 | 34.2 ± 2.0 | 35.3 ± 2.1 | 32.2 ± 1.0 | 28.5 ± 1.0 | 26.8 ± 1.2 | 52.7 ± 1.9 | 55.7 ± 3.0 | 66.5 ± 3.5 | |||||||
| S | 52.7 ± 1.5 | 52.0 ± 1.8 | 53.9 ± 1.2 | 56.4 ± 2.0 | 54.8 ± 2.4 | 7.1 ± 0.5 | 0 ± 1.2 | 0 ± 2.1 | |||||||
| G2/M | 33.1 ± 1.4 | 12.7 ± 0.9 | 13.9 ± 0.5 | 15.1 ± 1.2 | 18.4 ± 1.6 | 40.2 ± 0.6 | 44.3 ± 2.2 | 33.5 ± 2.4 | |||||||
| Sub-G1 | 3.5 ± 1.0 | 3.5 ± 0.3 | 6.1 ± 0.4 | 7.7 ± 0.6 | 6.5 ± 1.3 | 13.7 ± 1.2* | 32.2 ± 1.3 | 43.2 ± 1.5* | |||||||
| HL-60/Bcl-2 | |||||||||||||||
| G0/G1 | 37.4 ± 1.3 | 39.0 ± 2.1 | 33.5 ± 1.0 | 35.6 ± 1.7 | 38.4 ± 1.2 | 80.67 ± 1.7† | 81.3 ± 2.4 | 85.8 ± 2.4† | |||||||
| S | 56.5 ± 2.0 | 54.8 ± 1.9 | 54.5 ± 2.0 | 42.7 ± 1.8 | 33.7 ± 1.3 | 3.6 ± 1.4 | 3.1 ± 1.3 | 5.3 ± 1.5 | |||||||
| G2/M | 6.2 ± 0.5 | 6.2 ± 0.9 | 12.0 ± 2.2 | 22.0 ± 0.9 | 27.9 ± 1.2 | 15.9 ± 1.3 | 15.5 ± 1.2 | 8.9 ± 1.8 | |||||||
| Sub-G1 | 4.3 ± 0.4 | 4.3 ± 0.5 | 5.6 ± 1.5 | 6.1 ± 0.6 | 5.0 ± 0.4 | 6.5 ± 1.9 | 11.1 ± 1.3 | 16.8 ± 0.8 | |||||||
. | % of cells in designated cell phase at various hours . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell phase . | 0 . | 2 . | 4 . | 6 . | 8 . | 24 . | 32 . | 48 . | |||||||
| HL-60/Neo | |||||||||||||||
| G0/G1 | 34.2 ± 2.0 | 35.3 ± 2.1 | 32.2 ± 1.0 | 28.5 ± 1.0 | 26.8 ± 1.2 | 52.7 ± 1.9 | 55.7 ± 3.0 | 66.5 ± 3.5 | |||||||
| S | 52.7 ± 1.5 | 52.0 ± 1.8 | 53.9 ± 1.2 | 56.4 ± 2.0 | 54.8 ± 2.4 | 7.1 ± 0.5 | 0 ± 1.2 | 0 ± 2.1 | |||||||
| G2/M | 33.1 ± 1.4 | 12.7 ± 0.9 | 13.9 ± 0.5 | 15.1 ± 1.2 | 18.4 ± 1.6 | 40.2 ± 0.6 | 44.3 ± 2.2 | 33.5 ± 2.4 | |||||||
| Sub-G1 | 3.5 ± 1.0 | 3.5 ± 0.3 | 6.1 ± 0.4 | 7.7 ± 0.6 | 6.5 ± 1.3 | 13.7 ± 1.2* | 32.2 ± 1.3 | 43.2 ± 1.5* | |||||||
| HL-60/Bcl-2 | |||||||||||||||
| G0/G1 | 37.4 ± 1.3 | 39.0 ± 2.1 | 33.5 ± 1.0 | 35.6 ± 1.7 | 38.4 ± 1.2 | 80.67 ± 1.7† | 81.3 ± 2.4 | 85.8 ± 2.4† | |||||||
| S | 56.5 ± 2.0 | 54.8 ± 1.9 | 54.5 ± 2.0 | 42.7 ± 1.8 | 33.7 ± 1.3 | 3.6 ± 1.4 | 3.1 ± 1.3 | 5.3 ± 1.5 | |||||||
| G2/M | 6.2 ± 0.5 | 6.2 ± 0.9 | 12.0 ± 2.2 | 22.0 ± 0.9 | 27.9 ± 1.2 | 15.9 ± 1.3 | 15.5 ± 1.2 | 8.9 ± 1.8 | |||||||
| Sub-G1 | 4.3 ± 0.4 | 4.3 ± 0.5 | 5.6 ± 1.5 | 6.1 ± 0.6 | 5.0 ± 0.4 | 6.5 ± 1.9 | 11.1 ± 1.3 | 16.8 ± 0.8 | |||||||
More G1 arrest of HL-60/Bcl-2 versus HL-60/Neo cells is induced by 17-AAG. Cells were incubated with 2.0 μM 17-AAG for the indicated time interval and stained with propidium iodide, and the DNA content was assayed by flow cytometry. The percentage of cells in the various phases of cell cycle and the percentage of apoptotic cells containing subdiploid DNA content (sub-G1 fraction) were determined. Data represent the mean of ± SE of 3 experiments.
Values significantly greater (P<.05) than those observed in HL-60/Bcl-2 cells.
Values significantly greater (P<.05) than those observed in HL-60/Neo cells.