Kinetic analysis of G-CSFR mRNA expression in CD3+ T cells at single-cell level following G-CSF stimulation in vitro
Time, h . | After single stimulation of T cells (%) . | After single stimulation of PBMCs (%) . | After repetitive stimulation of T cells (%) . |
|---|---|---|---|
| 2 | 1/14 (7) | 2/12 (17) | — |
| 4 | 2/16 (12) | 3/18 (17) | — |
| 8 | 2/20 (10) | 4/17 (23) | — |
| 12 | 5/25 (20) | 5/17 (29) | — |
| 24 | 4/21 (19) | 4/21 (19) | 6/32 (19) |
| 36 | 0/21 (0) | — | 7/22 (32) |
| 48 | 0/22 (0) | — | 4/23 (17) |
| 60 | 0/23 (0) | — | 6/25 (24) |
| 72 | 0/18 (0) | 0/18 (0) | 4/24 (17) |
Time, h . | After single stimulation of T cells (%) . | After single stimulation of PBMCs (%) . | After repetitive stimulation of T cells (%) . |
|---|---|---|---|
| 2 | 1/14 (7) | 2/12 (17) | — |
| 4 | 2/16 (12) | 3/18 (17) | — |
| 8 | 2/20 (10) | 4/17 (23) | — |
| 12 | 5/25 (20) | 5/17 (29) | — |
| 24 | 4/21 (19) | 4/21 (19) | 6/32 (19) |
| 36 | 0/21 (0) | — | 7/22 (32) |
| 48 | 0/22 (0) | — | 4/23 (17) |
| 60 | 0/23 (0) | — | 6/25 (24) |
| 72 | 0/18 (0) | 0/18 (0) | 4/24 (17) |
Kinetic analysis of G-CSFR mRNA expression in CD3+ T cells at single-cell level following G-CSF stimulation in vitro. Direct stimulation of T cells (purity > 98%) compared with stimulation of PBMCs with G-CSF in vitro did not show a significant difference in the frequency of T cells expressing G-CSFR mRNA (P = .28, chi-square test). Repetitive stimulation of T cells every 24 hours led to sustained G-CSFR expression. All of the analyzed single cells expressed TCR-α chain mRNA. — indicates not determined