Cell division of naive CD4+ cells is inhibited by coculture with tolerized CD4+ cells
. | Number of cell divisions by CD45.2+ cells . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Culture . | 0 . | 1 . | 2 . | ≥3 . | |||
| Naive alone | 50.7% | 32.1% | 2.0% | 15.5% | |||
| Naive + tolerized | 75.1% | 22.2% | 1.1% | 2.1% | |||
. | Number of cell divisions by CD45.2+ cells . | . | . | . | |||
|---|---|---|---|---|---|---|---|
| Culture . | 0 . | 1 . | 2 . | ≥3 . | |||
| Naive alone | 50.7% | 32.1% | 2.0% | 15.5% | |||
| Naive + tolerized | 75.1% | 22.2% | 1.1% | 2.1% | |||
CFSE-labeled naive B6 CD45.2 CD4+ T cells were cultured with bm12 stimulators with or without the addition of tolerized B6 CD45.1 CD4+ cells at a ratio of 4 naive cells to 1 tolerized cell. On day 6, CD45.2 CD4+ cells were identified (Figure 2A) and analyzed for CFSE dilution by flow cytometry (Figure 2B). The number of cell divisions for each peak was assigned based on mean fluorescence intensity of undivided cells. Values are the percent of CD45.2 CD4+ cells that were present within the gate for each number of cell divisions. Data represent 1 of 2 reproducible experiments.