Table 3. Naturally occurring type I... | American Society of Hematology

Table 3.

Naturally occurring type I (IFN-γ) T cells (ELISPOT) recognizing 15- to 20-aa long peptides from the CDR regions of tumor-derived idiotypic immunoglobulin

Patient no.	HLA	Amino acid sequence	CDR region	Score†	Number of stimulated spots/10⁵ PBMCs, mean ± SEM‡
1	DRB10401	FCARVGRRAGWFVALWGHG	CDR3	12	15 ± 2.4
	DRB10401	IGYIYYTGTTHYNPSLKSRV	CDR2	22	12 ± 2.5
	DRB10401	GYSISDKYWTWIRQA	CDR1	9	< 10
2	DRB10401	FSLRTREMCVTWIRQ	CDR1	6	27 ± 11.9
	DRB10301	YCARIHRDNYYYAMDVWGQG	CDR3	20	26 ± 14.0
	DRB10401	YCARIHRDNYYYAMDVWGQG	CDR3	20
	DRB10301	LARIDWDDEKYYSTSLKTRLTI	CDR2	34	14 ± 2.1
	DRB10401	LARIDWDDEKYYSTSLKTRLTI	CDR2	22
5	DRB10401	TRDHHRLEWLSFHYYGMDV	CDR3	14	18 ± 2.3
	DRB10401	YIRSKTFGGTTEYAASVKG	CDR2	16	17 ± 1.8
	DRB10401	TRDHHRLEWLSFHYYG	CDR3	6	13 ± 0.9
	DRB10401	SGFTFGDYVMNWVRQAPG	CDR1	16	13 ± 2.3
	DRB10401	WVGYIRSKTFGGTTEY	CDR2	16	< 10
	DRB10401	GGTTEYAASVKGRFTI	CDR2	12	< 10
	DRB10401	SFHYYGMDVWGQGTTV	CDR3	16	< 10
	DRB10401	HYYGMDVWGQGTTVTT	CDR3	20	< 10

Patient no.	HLA	Amino acid sequence	CDR region	Score†	Number of stimulated spots/10⁵ PBMCs, mean ± SEM‡
1	DRB10401	FCARVGRRAGWFVALWGHG	CDR3	12	15 ± 2.4
	DRB10401	IGYIYYTGTTHYNPSLKSRV	CDR2	22	12 ± 2.5
	DRB10401	GYSISDKYWTWIRQA	CDR1	9	< 10
2	DRB10401	FSLRTREMCVTWIRQ	CDR1	6	27 ± 11.9
	DRB10301	YCARIHRDNYYYAMDVWGQG	CDR3	20	26 ± 14.0
	DRB10401	YCARIHRDNYYYAMDVWGQG	CDR3	20
	DRB10301	LARIDWDDEKYYSTSLKTRLTI	CDR2	34	14 ± 2.1
	DRB10401	LARIDWDDEKYYSTSLKTRLTI	CDR2	22
5	DRB10401	TRDHHRLEWLSFHYYGMDV	CDR3	14	18 ± 2.3
	DRB10401	YIRSKTFGGTTEYAASVKG	CDR2	16	17 ± 1.8
	DRB10401	TRDHHRLEWLSFHYYG	CDR3	6	13 ± 0.9
	DRB10401	SGFTFGDYVMNWVRQAPG	CDR1	16	13 ± 2.3
	DRB10401	WVGYIRSKTFGGTTEY	CDR2	16	< 10
	DRB10401	GGTTEYAASVKGRFTI	CDR2	12	< 10
	DRB10401	SFHYYGMDVWGQGTTV	CDR3	16	< 10
	DRB10401	HYYGMDVWGQGTTVTT	CDR3	20	< 10

aa's within the CDR region are indicated in bold.

†

Highest score found in the SYFPEITHI database.

‡

A specific T-cell response was defined as at least 10 spots/10⁵ PBMCs.

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