Table 2. Naturally occurring type I... | American Society of Hematology

Table 2.

Naturally occurring type I (IFN-γ) T cells (ELISPOT) recognizing 8- to 10-aa long peptides from the CDR regions of the tumor-derived idiotypic immunoglobulin

Patient no.	HLA-ABC genotype	Amino acid sequence	CDR region	Score†	Binding half-life, min‡	Number of stimulated spots/10⁵ PBMCs, mean ± SEM§
1	A*0201	ALWGHGTLV	CDR3	25	577.0	19 ± 6.0
	A*0201	SLKSRVSLSV	CDR2	24	2.2	18 ± 3.0
	A1	GLEWIGYIY	CDR2	27	90.0	17 ± 1.5
	B*4403	RVGRRAGWF	CDR3	—	0.15	17 ± 2.0
	A*0201	VGRRAGWFV	CDR3	11	2.4	16 ± 2.5
	B62	SGYSISDKY	CDR1	—	4.0	14 ± 2.5
	B*4403	LEWIGYIYY	CDR2	—	180.0	< 10
2	B8	SLKTRLTIS	CDR2	26	4.0	35 ± 14.0
	A1	RIDWDDEKY	CDR2	27	25.0	26 ± 0.5
	A1	HRDNYYYAM	CDR3	11	0.05	23 ± 10.0
	B8	SLRTREMCV	CDR1	22	24.0	24 ± 10.5
	B8	DEKYYSTSL	CDR2	21	0.8	< 10
	B8	STSLKTRL	CDR2	23	4.0	< 10
	A1	CARIHRDNY	CDR3	16	0.05	< 10
5	A3	RLEWLSFHY	CDR3	20	12.0	19 ± 1.7
	B7	CTRDHHRL	CDR3	—	—	< 10
	A3	RLEWLSFHYY	CDR3	20	12.0	< 10

Patient no.	HLA-ABC genotype	Amino acid sequence	CDR region	Score†	Binding half-life, min‡	Number of stimulated spots/10⁵ PBMCs, mean ± SEM§
1	A*0201	ALWGHGTLV	CDR3	25	577.0	19 ± 6.0
	A*0201	SLKSRVSLSV	CDR2	24	2.2	18 ± 3.0
	A1	GLEWIGYIY	CDR2	27	90.0	17 ± 1.5
	B*4403	RVGRRAGWF	CDR3	—	0.15	17 ± 2.0
	A*0201	VGRRAGWFV	CDR3	11	2.4	16 ± 2.5
	B62	SGYSISDKY	CDR1	—	4.0	14 ± 2.5
	B*4403	LEWIGYIYY	CDR2	—	180.0	< 10
2	B8	SLKTRLTIS	CDR2	26	4.0	35 ± 14.0
	A1	RIDWDDEKY	CDR2	27	25.0	26 ± 0.5
	A1	HRDNYYYAM	CDR3	11	0.05	23 ± 10.0
	B8	SLRTREMCV	CDR1	22	24.0	24 ± 10.5
	B8	DEKYYSTSL	CDR2	21	0.8	< 10
	B8	STSLKTRL	CDR2	23	4.0	< 10
	A1	CARIHRDNY	CDR3	16	0.05	< 10
5	A3	RLEWLSFHY	CDR3	20	12.0	19 ± 1.7
	B7	CTRDHHRL	CDR3	—	—	< 10
	A3	RLEWLSFHYY	CDR3	20	12.0	< 10

aa's within the CDR region are indicated in bold. — indicates not available.

†

SYFPEITHI.

‡

BIMAS.

A specific T-cell response was defined as at least 10 spots/10⁵ PBMCs.

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