Fcγ receptor expression and phagocytosis in COS-1 cells transiently cotransfected with CD8/γ chain chimeras and FcγRIIIA
. | Expression (MFI) . | . | . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | FcγRIII . | . | . | CD8 . | . | . | Phagocytosis (PI) . | . | . | ||||||
| Experiment no. . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | ||||||
| FcγRIIIA | |||||||||||||||
| +CD8-γ-γ | 50 | 74 | 29 | 163 | 109 | 96 | 10 | 23 | 17 | ||||||
| +CD8-CD8-γ | 4 | 14 | 1 | 341 | 222 | 306 | 0 | 0 | 0 | ||||||
| +CD8-γ (Cys7/Asp11)-γ | 4 | 7 | 1 | 264 | 232 | 160 | 0 | 7 | 0 | ||||||
. | Expression (MFI) . | . | . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | FcγRIII . | . | . | CD8 . | . | . | Phagocytosis (PI) . | . | . | ||||||
| Experiment no. . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | ||||||
| FcγRIIIA | |||||||||||||||
| +CD8-γ-γ | 50 | 74 | 29 | 163 | 109 | 96 | 10 | 23 | 17 | ||||||
| +CD8-CD8-γ | 4 | 14 | 1 | 341 | 222 | 306 | 0 | 0 | 0 | ||||||
| +CD8-γ (Cys7/Asp11)-γ | 4 | 7 | 1 | 264 | 232 | 160 | 0 | 7 | 0 | ||||||
The structure of the CD8/γ chain chimeras is indicated in Figure 2. Three representative experiments are shown. Cell surface expression of transfected receptors, determined by flow cytometry using mAb 3G8 for FcγRIIIA, and anti-CD8 antibody for the CD8/γ chimeras, is presented as mean fluorescence intensity (MFI). For each experiment, 900-1000 cells were microscopically examined in a random fashion to determine the phagocytic index (PI).