Quantification and characterization of brain-sequestered leukocytes of naive, CM, NCM, WT, and KO mice
. | . | . | . | T lymphocytes . | . | |
|---|---|---|---|---|---|---|
| Mice* . | Leukocytes† . | Macrophages . | Neutrophils . | CD3+ CD4+ . | CD3+ CD8+ . | |
| WT | ||||||
| Naive | 138.1 ± 17.8 | 66.4 ± 11.6 | 14.7 ± 5.1 | 6.9 ± 3.05 | 6.3 ± 2.5 | |
| NCM | 180.5 ± 36.9 | 136.1 ± 30.5 | 28.5 ± 5.2 | 4.5 ± 0.9 | 12.4 ± 4.9 | |
| CM | 697 ± 73‡§ | 278.8 ± 27.8‡ | 240 ± 38.2‡§ | 41.1 ± 10.2‡§ | 190.3 ± 64.1‡§ | |
| KO | ||||||
| Naive | 231.3 ± 37.5 | 99.6 ± 22.9 | 37.5 ± 10.5 | 4.7 ± 1 | 3.5 ± 0.5 | |
| NCM | 310.8 ± 48.6 | 190.1 ± 42.5 | 82.7 ± 17.5 | 10 ± 5.3 | 4.5 ± 1.9 | |
| CM | 619.1 ± 87.8∥¶ | 239.5 ± 66.6∥ | 183.2 ± 43.7∥ | 33.6 ± 5∥ | 181.1 ± 31.3∥¶ | |
. | . | . | . | T lymphocytes . | . | |
|---|---|---|---|---|---|---|
| Mice* . | Leukocytes† . | Macrophages . | Neutrophils . | CD3+ CD4+ . | CD3+ CD8+ . | |
| WT | ||||||
| Naive | 138.1 ± 17.8 | 66.4 ± 11.6 | 14.7 ± 5.1 | 6.9 ± 3.05 | 6.3 ± 2.5 | |
| NCM | 180.5 ± 36.9 | 136.1 ± 30.5 | 28.5 ± 5.2 | 4.5 ± 0.9 | 12.4 ± 4.9 | |
| CM | 697 ± 73‡§ | 278.8 ± 27.8‡ | 240 ± 38.2‡§ | 41.1 ± 10.2‡§ | 190.3 ± 64.1‡§ | |
| KO | ||||||
| Naive | 231.3 ± 37.5 | 99.6 ± 22.9 | 37.5 ± 10.5 | 4.7 ± 1 | 3.5 ± 0.5 | |
| NCM | 310.8 ± 48.6 | 190.1 ± 42.5 | 82.7 ± 17.5 | 10 ± 5.3 | 4.5 ± 1.9 | |
| CM | 619.1 ± 87.8∥¶ | 239.5 ± 66.6∥ | 183.2 ± 43.7∥ | 33.6 ± 5∥ | 181.1 ± 31.3∥¶ | |
PbA-infected mice were killed between day 7 and day 10 after infection, when mice displayed neurologic symptoms. Mice without CM were killed at day 10.
Results are mean ± SEM (× 103) of 3 to 9 mice per group.
P < .05 versus naive WT mice.
P < .05 versus NCM WT mice.
P < .05 versus naive KO mice.
P < .05 versus NCM KO mice.