Level of proviral DNA and integrated virus found within myDCs, pcDCs, and T lymphocytes
. | HIV-1 plasma RNA virus load . | CD4 T cells, per μL . | Contaminating T cells, % . | . | Lowest no. of cells giving positive signal . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Patients . | . | . | pcDCs . | myDCs . | pcDCs . | myDCs . | T cells . | |||
| pt09 | 20 762 | 615 | BDL | BDL | < 5 000 | 5 000 | 5 000 | |||
| pt77 | 380 857 | 83 | 0.38 | 0.99 | 1 000 | 1 000* | 40 | |||
| pt78 | 62 032 | 651 | 0.19 | 0.12 | 5 000 | 1 000 | 1 000 | |||
| pt85 | 51 666 | 48 | BDL | BDL | 5 000 | 1 000 | 5 000 | |||
| pt95 | 14 028 | 308 | 0.41 | 0.29 | < 5 000 | < 5 000 | 200 | |||
| pt96 | 68 433 | 157 | 0.94 | 0.23 | 5 000 | 200* | 1 000 | |||
| pt97 | 174 782 | 110 | 0.94 | BDL | 1 000 | 200 | 200 | |||
| pt98 | 1 600 | 271 | BDL | BDL | 5 000 | 1 000 | 200 | |||
| pt05 | 500 000 | 114 | BDL | BDL | 1 000 | 40 | 40 | |||
| pt07 | 280 500 | 193 | 0.39 | BDL | 5 000 | 5 000 | 5 000 | |||
| pt06 | 84 531 | 191 | BDL | BDL | 1 000 | 1 000 | 1 000 | |||
| pt02 | 4 238 | 884 | 0.29 | 0.08 | 5 000 | 5 000 | 5 000 | |||
| pt10 | 390 158 | 213 | 0.88 | 0.46 | 200 | 200* | 40 | |||
| pt11 | 105 092 | 174 | 0.33 | BDL | 200 | 1 000* | 40* | |||
. | HIV-1 plasma RNA virus load . | CD4 T cells, per μL . | Contaminating T cells, % . | . | Lowest no. of cells giving positive signal . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Patients . | . | . | pcDCs . | myDCs . | pcDCs . | myDCs . | T cells . | |||
| pt09 | 20 762 | 615 | BDL | BDL | < 5 000 | 5 000 | 5 000 | |||
| pt77 | 380 857 | 83 | 0.38 | 0.99 | 1 000 | 1 000* | 40 | |||
| pt78 | 62 032 | 651 | 0.19 | 0.12 | 5 000 | 1 000 | 1 000 | |||
| pt85 | 51 666 | 48 | BDL | BDL | 5 000 | 1 000 | 5 000 | |||
| pt95 | 14 028 | 308 | 0.41 | 0.29 | < 5 000 | < 5 000 | 200 | |||
| pt96 | 68 433 | 157 | 0.94 | 0.23 | 5 000 | 200* | 1 000 | |||
| pt97 | 174 782 | 110 | 0.94 | BDL | 1 000 | 200 | 200 | |||
| pt98 | 1 600 | 271 | BDL | BDL | 5 000 | 1 000 | 200 | |||
| pt05 | 500 000 | 114 | BDL | BDL | 1 000 | 40 | 40 | |||
| pt07 | 280 500 | 193 | 0.39 | BDL | 5 000 | 5 000 | 5 000 | |||
| pt06 | 84 531 | 191 | BDL | BDL | 1 000 | 1 000 | 1 000 | |||
| pt02 | 4 238 | 884 | 0.29 | 0.08 | 5 000 | 5 000 | 5 000 | |||
| pt10 | 390 158 | 213 | 0.88 | 0.46 | 200 | 200* | 40 | |||
| pt11 | 105 092 | 174 | 0.33 | BDL | 200 | 1 000* | 40* | |||
Provirus DNA detected by 5-fold limiting-dilution PCR with DNA from 5 000, 1 000, 200, 40, or 8 cells per reaction. The lowest concentration of cells giving a positive PCR band is indicated. Fewer than 5 000 indicates undetectable proviral DNA. The level of contaminating T lymphocytes was determined by flow cytometric analysis as shown in Figure 1A. Integrated virus was measured by means of an Alu-LTR nested PCR technique, with the use of the equivalent of 5 000 cells. All samples were assessed for level of integrated virus. pt indicates patient; BDL, below detection limit (ie, that no T lymphocytes were detected in the top left-hand quadrant of the cocktail/HLA-DR plot).
Integrated virus was detected in this sample.