PCR primers and reaction parameters
| SNP no. . | Chromosome location . | Forward primer . | Reverse primer . | Sequencing primer . | SNP sequence . | Magnesium concentration, mM . | Annealing temperature, °C . | Allele frequency, % (n) . |
|---|---|---|---|---|---|---|---|---|
| 8132* | 4q28 | Biotin-GGGCTCCTGGCTCTTT | CCTGTCCCTCTGTGTCAACC | TCATAGGCTCTCACT | GAGG/AAGT | 3 | 52-60 | 44 (90) |
| 2717* | 5q32 | CGCTTACCTGCCAGACTGC | Biotin-GGCCAGGACGATGAGAGA | CCACGACGTCACGCAG | CAGC/GAAA | 3 | 60 | 35 (88) |
| 2301* | 6q25 | Biotin-CGGGCTGTGCTTTCTCGT | CCGTACTCGTACGGCAGGTC | GCCCAGATACCCCAAA | CGGC/TTTT | 3 | 56 | 50 (74) |
| HA-243 | 7p13 | Biotin-GGCCGCATCTACACCTACAT | CGTTGGCCACAGCATAGAGA | CTCCTGGTAGGGGTTC | TCCG/ATGA | 2 | 58 | 26 (44) |
| 8111* | 7q36 | CCAGATGCCCAGCTAGT | Biotin-ACTCTCCAGGCACTTCAGG | CCAGCCCCTCAGATG | ATGA/GCAC | 3 | 52-60 | 33 (87) |
| 1791* | 8p22 | Biotin-TTCAAGGCTCTGTCAGTG | AAGCAAAAACAGAAGAACAA | ACAACAACAAAACCCCACAG | TAGT/CAGC | 3 | 52 | 48 (87) |
| HA-844 | 9q22 | ATTTAGAGGGGTTTGATTGTT | Biotin-AAGTTCTAATTTTTCTGGCTGT | TTGCAGTCAGCAGATCAC | ACCG/CAAC | 2 | 58 | 39 (74) |
| 8097* | 14q32 | Biotin-GAGCGGAGAGCGAAGGTGG | GTGGCGGCGGGAAC | TTGAAGGGCTGGGTA | TCAT/CTAC | 3 | 60 | 43 (67) |
| 8102* | 16q13 | TCTAACATCATGGCCGACTT | Biotin-GAAGAGTGACAGTCCCAAGG | CAAGGGCTGGTGAGTG | GTGT/CGTT | 3 | 54 | 38 (68) |
| CD3145531* | 17q23 | Biotin-TGTGAACAACAAAGAGAAAACC | GCTGTTATTCACGCCACTG | CTCACCTTCCACCAAC | CAGG/CTGT | 2 | 58 | 49 (75) |
| CD314519203* | 17q23 | Biotin-GATCTGGTCCCATCACCTATA | GGCGTGGTTGGCTCTGTTGAA | TCTCCCTCCTGTTCCT | CTAA/GCAA | 2 | 58 | 48 (74) |
| HA-1383474* | 19p13 | Biotin-TCGCTGAGGGCCTTGAGAAA | CTTGGGTCTGGCTCTGTCTT | GTGGCTCTCACCGTC | TGCG/ATGA | 2 | 58 | 37 (72) |
| 8049* | 20q13 | CGCCATGAGCAACCTG | Biotin-CGGGAGGGAAGTCAAAGTCA | CAGAGTGGACTACAT | CATT/CCTG | 3 | 60 | 48 (65) |
| 2384* | 22q13 | Biotin-GGCTGCCATTATGACCAACTA | TCCCCACTACTCTCAGAAA | GAAACTTCTTGAACCCA | TAAC/TTGG | 3 | 58 | 29 (67) |
| SNP no. . | Chromosome location . | Forward primer . | Reverse primer . | Sequencing primer . | SNP sequence . | Magnesium concentration, mM . | Annealing temperature, °C . | Allele frequency, % (n) . |
|---|---|---|---|---|---|---|---|---|
| 8132* | 4q28 | Biotin-GGGCTCCTGGCTCTTT | CCTGTCCCTCTGTGTCAACC | TCATAGGCTCTCACT | GAGG/AAGT | 3 | 52-60 | 44 (90) |
| 2717* | 5q32 | CGCTTACCTGCCAGACTGC | Biotin-GGCCAGGACGATGAGAGA | CCACGACGTCACGCAG | CAGC/GAAA | 3 | 60 | 35 (88) |
| 2301* | 6q25 | Biotin-CGGGCTGTGCTTTCTCGT | CCGTACTCGTACGGCAGGTC | GCCCAGATACCCCAAA | CGGC/TTTT | 3 | 56 | 50 (74) |
| HA-243 | 7p13 | Biotin-GGCCGCATCTACACCTACAT | CGTTGGCCACAGCATAGAGA | CTCCTGGTAGGGGTTC | TCCG/ATGA | 2 | 58 | 26 (44) |
| 8111* | 7q36 | CCAGATGCCCAGCTAGT | Biotin-ACTCTCCAGGCACTTCAGG | CCAGCCCCTCAGATG | ATGA/GCAC | 3 | 52-60 | 33 (87) |
| 1791* | 8p22 | Biotin-TTCAAGGCTCTGTCAGTG | AAGCAAAAACAGAAGAACAA | ACAACAACAAAACCCCACAG | TAGT/CAGC | 3 | 52 | 48 (87) |
| HA-844 | 9q22 | ATTTAGAGGGGTTTGATTGTT | Biotin-AAGTTCTAATTTTTCTGGCTGT | TTGCAGTCAGCAGATCAC | ACCG/CAAC | 2 | 58 | 39 (74) |
| 8097* | 14q32 | Biotin-GAGCGGAGAGCGAAGGTGG | GTGGCGGCGGGAAC | TTGAAGGGCTGGGTA | TCAT/CTAC | 3 | 60 | 43 (67) |
| 8102* | 16q13 | TCTAACATCATGGCCGACTT | Biotin-GAAGAGTGACAGTCCCAAGG | CAAGGGCTGGTGAGTG | GTGT/CGTT | 3 | 54 | 38 (68) |
| CD3145531* | 17q23 | Biotin-TGTGAACAACAAAGAGAAAACC | GCTGTTATTCACGCCACTG | CTCACCTTCCACCAAC | CAGG/CTGT | 2 | 58 | 49 (75) |
| CD314519203* | 17q23 | Biotin-GATCTGGTCCCATCACCTATA | GGCGTGGTTGGCTCTGTTGAA | TCTCCCTCCTGTTCCT | CTAA/GCAA | 2 | 58 | 48 (74) |
| HA-1383474* | 19p13 | Biotin-TCGCTGAGGGCCTTGAGAAA | CTTGGGTCTGGCTCTGTCTT | GTGGCTCTCACCGTC | TGCG/ATGA | 2 | 58 | 37 (72) |
| 8049* | 20q13 | CGCCATGAGCAACCTG | Biotin-CGGGAGGGAAGTCAAAGTCA | CAGAGTGGACTACAT | CATT/CCTG | 3 | 60 | 48 (65) |
| 2384* | 22q13 | Biotin-GGCTGCCATTATGACCAACTA | TCCCCACTACTCTCAGAAA | GAAACTTCTTGAACCCA | TAAC/TTGG | 3 | 58 | 29 (67) |
SNP numbers are from the HGBASE SNP database athttp://hgbase.cgr.ki.se/.16 For example, SNP 8132 is HGBASE:SNP000008132.