Cytokine secretion by immature MoDCs
| Immature MoDC . | IL-1β† . | IL-6* . | IL-10† . | IL-12p70† . | n . |
|---|---|---|---|---|---|
| Control | 174 ± 44 | 4.2 ± 1.1 | 316 ± 41 | 0 ± 0 | 7-9 |
| TNF-α + IFN-α + PGE2 | 146 ± 39 | 4.7 ± 1.2 | 340 ± 48 | 0 ± 0 | 5-8 |
| CD40L | 166 ± 26 | 10.6 ± 2.8 | 1 299 ± 390 | 4 930 ± 3 152 | 5-6 |
| CD40L + IFN-γ | 224 ± 41 | 19.7 ± 5.5 | 3 779 ± 1 132 | 17 799 ± 10 272 | 7-8 |
| E coli | 3 024 ± 1 060 | 28.4 ± 7.0 | 12 713 ± 5 128 | 15 335 ± 10 062 | 6-9 |
| Immature MoDC . | IL-1β† . | IL-6* . | IL-10† . | IL-12p70† . | n . |
|---|---|---|---|---|---|
| Control | 174 ± 44 | 4.2 ± 1.1 | 316 ± 41 | 0 ± 0 | 7-9 |
| TNF-α + IFN-α + PGE2 | 146 ± 39 | 4.7 ± 1.2 | 340 ± 48 | 0 ± 0 | 5-8 |
| CD40L | 166 ± 26 | 10.6 ± 2.8 | 1 299 ± 390 | 4 930 ± 3 152 | 5-6 |
| CD40L + IFN-γ | 224 ± 41 | 19.7 ± 5.5 | 3 779 ± 1 132 | 17 799 ± 10 272 | 7-8 |
| E coli | 3 024 ± 1 060 | 28.4 ± 7.0 | 12 713 ± 5 128 | 15 335 ± 10 062 | 6-9 |
IL-6 in ng/mL.
Concentration of cytokines in pg/mL.
MoDCs were prepared by culturing purified CD14+ monocytes for 7 days in GM-CSF and IL-4. On day 7, the cultures were pooled, adjusted to 1 × 105 DC/mL, and split into replicate wells. The indicated stimuli were then added for 3 days, and supernatants were examined for cytokines by ELISAs. Shown are the means ± SEM of n individual experiments.