IFN-γ does not shift immature DCs toward macrophages
| . | No cytokine . | GM-CSF + IL-4 . | |||
|---|---|---|---|---|---|
| None . | IFN-γ . | IFN-γ + M-CSF . | M-CSF + IL-6 . | ||
| CD1153-150 | ND | 76 ± 10 | 83 ± 13 | 10 ± 3 | 15 ± 2 |
| CD1a3-151 | 11 ± 2 | 310 ± 35 | 298 ± 29 | 358 ± 42 | 399 ± 50 |
| CD143-151 | 56 ± 9 | < 5 | < 5 | < 5 | < 5 |
| CD833-152 | < 5% | 99% | 98% | 99% | 99% |
| MLR3-153 | ND | 2 ± 0.1 | 5 ± 0.8 | ND | ND |
| MLR3-155 | ND | 22 ± 2 | 24 ± 3 | ND | ND |
| . | No cytokine . | GM-CSF + IL-4 . | |||
|---|---|---|---|---|---|
| None . | IFN-γ . | IFN-γ + M-CSF . | M-CSF + IL-6 . | ||
| CD1153-150 | ND | 76 ± 10 | 83 ± 13 | 10 ± 3 | 15 ± 2 |
| CD1a3-151 | 11 ± 2 | 310 ± 35 | 298 ± 29 | 358 ± 42 | 399 ± 50 |
| CD143-151 | 56 ± 9 | < 5 | < 5 | < 5 | < 5 |
| CD833-152 | < 5% | 99% | 98% | 99% | 99% |
| MLR3-153 | ND | 2 ± 0.1 | 5 ± 0.8 | ND | ND |
| MLR3-155 | ND | 22 ± 2 | 24 ± 3 | ND | ND |
Day 5 immature DCs were cultured in the absence of cytokine or in GM-CSF plus IL-4 in the absence or presence of 25 ng/mL IFN-γ, 25 ng/mL IFN-γ plus 100 ng/mL M-CSF, or 100 ng/mL M-CSF plus 100 ng/mL IL-6.
ND indicates not done.
After 1 day, membrane CD115 expression was analyzed by FACS.
After 3 days, CD1a and CD14 expression were analyzed by FACS. For CD1a, CD14, and CD115, results are expressed in MFI ± values, mean SD of 4 separate experiments.
After 3 days, cells were further stimulated for 24 hours with LPS, and CD83 was analyzed by FACS. Results are expressed in percentage of positive cells.
After 2 days, cells were used as stimulatory cells in primary allogeneic MLRs.
After 2 days, cells were stimulated for 24 hours with LPS before being used as stimulatory cells in primary allogeneic MLRs. Results are expressed in counts per minute × 10−3 as mean ± SD of quadruplicate values and are representative of 1 of 3 experiments.