IFN-γ prevents murine progenitor differentiation into DCs
| . | None . | IFN-γ . |
|---|---|---|
| Surface markers | ||
| CD11b | 857 ± 75 | 320 ± 95 |
| CD11c | 536 ± 45 | 4 ± 0.2 |
| CD86 | 136 ± 23 | 33 ± 9 |
| F4/80 | 140 ± 19 | 60 ± 10 |
| IAb | 49% ± 10, 978 ± 83* | 8 ± 1.2 |
| 61% ± 22, 59 ± 13* | ||
| Allogenic MLR | 41 ± 9 | 4 ± 0.8 |
| . | None . | IFN-γ . |
|---|---|---|
| Surface markers | ||
| CD11b | 857 ± 75 | 320 ± 95 |
| CD11c | 536 ± 45 | 4 ± 0.2 |
| CD86 | 136 ± 23 | 33 ± 9 |
| F4/80 | 140 ± 19 | 60 ± 10 |
| IAb | 49% ± 10, 978 ± 83* | 8 ± 1.2 |
| 61% ± 22, 59 ± 13* | ||
| Allogenic MLR | 41 ± 9 | 4 ± 0.8 |
Bone marrow cells from C57BL/6 mice were cultured in CM containing 5 ng/mL GM-CSF in the absence or presence of 25 ng/mL IFN-γ. After 5 days, phenotype was analyzed by FACS. In the absence or presence of IFN-γ, cells were gated on CD11c+or on Gr1− cells, respectively, as described in “Materials and methods.” For all the markers, excepted for IAb, homogeneous populations were obtained and results are expressed in MFI values (mean ± SD, n = 4).
For IAb, two populations expressing high and low levels of IAb were observed. The percentage of cells in each population (left values; mean percent ± SD, n = 4) and the MFI values of each population (right values; mean ± SD, n = 4) are mentioned. At day 5, cells were used as stimulatory cells in primary allogenic MLRs. Results are expressed in counts per minute × 10−3 as mean ± SD of quadruplicate values and are representative of 1 of 3 experiments.