Characteristics of monocytes cultured for 5 days with GM-CSF plus IL-4, GM-CSF, or M-CSF in the absence or presence of IFN-γ
| . | GM-CSF + IL-4 . | GM-CSF . | M-CSF . | |||
|---|---|---|---|---|---|---|
| − IFN-γ . | + IFN-γ . | − IFN-γ . | + IFN-γ . | − IFN-γ . | + IFN-γ . | |
| Markers | ||||||
| CD1a | 320 | 70 | 36 | 16 | 27 | 18 |
| CD14 | 5 | 8 | 590 | 43 | 370 | 89 |
| CD64 | 4 | 112 | 58 | 309 | 13 | 33 |
| CD86 | 8 | 76 | 18 | 24 | 12 | 22 |
| CD83 | 3 | 3 | 3 | 3 | 3 | 3 |
| CD83* | 130 | 3 | 3 | 3 | 3 | 3 |
| MHC-I | 335 | 1112 | 744 | 856 | 137 | 250 |
| HLA-DR | 539 | 1115 | 319 | 502 | 186 | 302 |
| RFD7 | 3 | 3 | 290 | 37 | 39 | 3 |
| MR | 271 | 175 | 160 | 51 | 320 | 100 |
| Endocytic activity | ||||||
| Dextran | 395 | 142 | 131 | 22 | 80 | 13 |
| Staphylococcus aureus | 34% | 7% | ND | ND | 90% | 10% |
| Latex beads | 0% | 3% | ND | ND | 73% | 13% |
| Enzymatic activity | ||||||
| Nonspecific esterase | − | +/− | ND | ND | ++ | +/− |
| Morphology | ||||||
| Nonadherent round/ovoid cells | + | − | + | − | + | − |
| Network of elongated and adherent cells | − | + | − | + | − | + |
| Nonadherent round/ovoid cells* | − | − | + | − | + | − |
| Network of elongated and adherent cells* | − | ++ | − | + | − | + |
| Ovoid and veils* | + | − | − | − | − | − |
| . | GM-CSF + IL-4 . | GM-CSF . | M-CSF . | |||
|---|---|---|---|---|---|---|
| − IFN-γ . | + IFN-γ . | − IFN-γ . | + IFN-γ . | − IFN-γ . | + IFN-γ . | |
| Markers | ||||||
| CD1a | 320 | 70 | 36 | 16 | 27 | 18 |
| CD14 | 5 | 8 | 590 | 43 | 370 | 89 |
| CD64 | 4 | 112 | 58 | 309 | 13 | 33 |
| CD86 | 8 | 76 | 18 | 24 | 12 | 22 |
| CD83 | 3 | 3 | 3 | 3 | 3 | 3 |
| CD83* | 130 | 3 | 3 | 3 | 3 | 3 |
| MHC-I | 335 | 1112 | 744 | 856 | 137 | 250 |
| HLA-DR | 539 | 1115 | 319 | 502 | 186 | 302 |
| RFD7 | 3 | 3 | 290 | 37 | 39 | 3 |
| MR | 271 | 175 | 160 | 51 | 320 | 100 |
| Endocytic activity | ||||||
| Dextran | 395 | 142 | 131 | 22 | 80 | 13 |
| Staphylococcus aureus | 34% | 7% | ND | ND | 90% | 10% |
| Latex beads | 0% | 3% | ND | ND | 73% | 13% |
| Enzymatic activity | ||||||
| Nonspecific esterase | − | +/− | ND | ND | ++ | +/− |
| Morphology | ||||||
| Nonadherent round/ovoid cells | + | − | + | − | + | − |
| Network of elongated and adherent cells | − | + | − | + | − | + |
| Nonadherent round/ovoid cells* | − | − | + | − | + | − |
| Network of elongated and adherent cells* | − | ++ | − | + | − | + |
| Ovoid and veils* | + | − | − | − | − | − |
Monocytes were cultured in medium containing GM-CSF plus IL-4, GM-CSF, or M-CSF in the absence or presence of 25 ng/mL IFN-γ. After 5 days, the phenotype and the uptake of FITC-labeled dextran, S aureus or latex beads was analyzed by FACS and cell morphology was determined by microscopy. Results are expressed in MFI values excepted the uptake of S aureus and latex beads expressed in percentage of positive cells. Results are representative of 1 of 5 experiments.
ND indicates not done; −, +/−, +, and ++, respectively, indicate absent, slightly detectable, detectable, and strongly evidenced.
CD83 expression and cell morphology were analyzed after 24 hours' incubation with 20 ng/mL LPS.