Variations of cAMP levels by PDE inhibitors
| Time . | Medium . | Sildenafil (50 μg/mL) . | Vardenafil (20 μg/mL) . | Vardenafil (50 μg/mL) . | Theophylline (60 μg/mL) . |
|---|---|---|---|---|---|
| 30 min | 24.4 | 24.4 | 28.7 | 28.1 | 1423 |
| 60 min | 20.0 | 24.6 | 14.9 | 18.6 | 1340 |
| 24 h | 23.5 | 20.5 | 24.8 | 21.4 | 1340 |
| Time . | Medium . | Sildenafil (50 μg/mL) . | Vardenafil (20 μg/mL) . | Vardenafil (50 μg/mL) . | Theophylline (60 μg/mL) . |
|---|---|---|---|---|---|
| 30 min | 24.4 | 24.4 | 28.7 | 28.1 | 1423 |
| 60 min | 20.0 | 24.6 | 14.9 | 18.6 | 1340 |
| 24 h | 23.5 | 20.5 | 24.8 | 21.4 | 1340 |
CLL PBMCs (1 × 106/mL) were incubated with the various PDE inhibitors for the indicated times. Total cellular cAMP (fmol/well) was measured, as described in “Patients, materials, and methods.”