Effect of oxidized EPA on expression of endothelial adhesion molecules
| . | Vehicle . | Vehicle + LPS . | EPA (100 μM) + LPS . | oxEPA (10 μM) + LPS . | oxEPA (50 μM) + LPS . | oxEPA (100 μM) + LPS . |
|---|---|---|---|---|---|---|
| ICAM-1 | 54 ± 5 | 272 ± 40 | 373 ± 36 | 265 ± 71 | 182 ± 50 | 61 ± 15* |
| VCAM-1 | 6 ± 4 | 211 ± 17 | 312 ± 38 | 198 ± 16 | 92 ± 26 | 29 ± 10† |
| E-selectin | ND | 248 ± 11 | 328 ± 75 | 191 ± 27 | 138 ± 36 | 53 ± 6† |
| Endoglin | 911 ± 65 | 736 ± 107 | 907 ± 45 | 781 ± 48 | 840 ± 38 | 743 ± 16 |
| HLA class I | 76 ± 7 | 79 ± 9 | 151 ± 16 | 67 ± 11 | 121 ± 31 | 109 ± 25 |
| . | Vehicle . | Vehicle + LPS . | EPA (100 μM) + LPS . | oxEPA (10 μM) + LPS . | oxEPA (50 μM) + LPS . | oxEPA (100 μM) + LPS . |
|---|---|---|---|---|---|---|
| ICAM-1 | 54 ± 5 | 272 ± 40 | 373 ± 36 | 265 ± 71 | 182 ± 50 | 61 ± 15* |
| VCAM-1 | 6 ± 4 | 211 ± 17 | 312 ± 38 | 198 ± 16 | 92 ± 26 | 29 ± 10† |
| E-selectin | ND | 248 ± 11 | 328 ± 75 | 191 ± 27 | 138 ± 36 | 53 ± 6† |
| Endoglin | 911 ± 65 | 736 ± 107 | 907 ± 45 | 781 ± 48 | 840 ± 38 | 743 ± 16 |
| HLA class I | 76 ± 7 | 79 ± 9 | 151 ± 16 | 67 ± 11 | 121 ± 31 | 109 ± 25 |
HUVECs were pretreated for 1 hour with vehicle or native (EPA) or oxidized EPA (oxEPA) prior to stimulation with LPS for 5 hours. Surface expression of adhesion molecules was quantitated by fluorescent immunoassay using appropriate monoclonal antibodies.
ND indicates none detected.
P < .006 and
P< .002 compared to Vehicle + LPS (n = 3).