Cell viability, growth, expandability of hematopoietic progenitor cells, and cytotoxicity of NK cells from peripheral blood progenitor cell collections after passages through an x-ray scan device
| Parameter . | Number of passages through x-ray scan . | ||||
|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 5 . | 10 . | |
| Viability (n = 11) | 98 ± 1 | 97 ± 1 | 96 ± 1 | 96 ± 2 | 97 ± 1 |
| CFC (n = 11) | 98 ± 6 | 103 ± 7 | 95 ± 9 | 106 ± 13 | 109 ± 10 |
| BFU-E (n = 11) | 104 ± 10 | 109 ± 17 | 103 ± 20 | 118 ± 28 | 118 ± 23 |
| CFU-GM (n = 11) | 98 ± 7 | 112 ± 8 | 94 ± 7 | 106 ± 8 | 111 ± 8 |
| CFU-Meg (n = 7) | — | — | — | 146 ± 40 | — |
| LTC-IC (n = 11) | 102 ± 12 | 100 ± 15 | 121 ± 31 | 132 ± 23 | 108 ± 22 |
| MNC expansion (n = 3) | — | — | — | 100 ± 12 | — |
| CFC expansion (n = 3) | — | — | — | 94 ± 19 | — |
| T-cell expansion (n = 4) | 104 ± 4 | — | — | 104 ± 4 | 95 ± 2 |
| NK cell expansion (n = 4) | 101 ± 8 | — | — | 98 ± 3 | 103 ± 6 |
| NK cell cytotoxicity (n = 3) | 97 ± 7 | — | — | 101 ± 10 | 107 ± 7 |
| Parameter . | Number of passages through x-ray scan . | ||||
|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 5 . | 10 . | |
| Viability (n = 11) | 98 ± 1 | 97 ± 1 | 96 ± 1 | 96 ± 2 | 97 ± 1 |
| CFC (n = 11) | 98 ± 6 | 103 ± 7 | 95 ± 9 | 106 ± 13 | 109 ± 10 |
| BFU-E (n = 11) | 104 ± 10 | 109 ± 17 | 103 ± 20 | 118 ± 28 | 118 ± 23 |
| CFU-GM (n = 11) | 98 ± 7 | 112 ± 8 | 94 ± 7 | 106 ± 8 | 111 ± 8 |
| CFU-Meg (n = 7) | — | — | — | 146 ± 40 | — |
| LTC-IC (n = 11) | 102 ± 12 | 100 ± 15 | 121 ± 31 | 132 ± 23 | 108 ± 22 |
| MNC expansion (n = 3) | — | — | — | 100 ± 12 | — |
| CFC expansion (n = 3) | — | — | — | 94 ± 19 | — |
| T-cell expansion (n = 4) | 104 ± 4 | — | — | 104 ± 4 | 95 ± 2 |
| NK cell expansion (n = 4) | 101 ± 8 | — | — | 98 ± 3 | 103 ± 6 |
| NK cell cytotoxicity (n = 3) | 97 ± 7 | — | — | 101 ± 10 | 107 ± 7 |
Values are percentage of controls, which are set to 100%, ± SEM.
CFCs indicates colony-forming cells; BFU-Es, erythroid burst-forming units; CFU-GMs, granulocyte-macrophage colony-forming units; CFU-Megs, megakaryocyte colony-forming units; LTC-ICs, long-term culture-initiating cells; MNC, mononuclear cell; NK, natural killer.