Primers used in expression analyses, methylation analyses by real-time polymerase chain reaction, and methylation analyses by bisulfite sequencing
| Expression analysis . | Methylation analysis, real-time polymerase chain reaction . | Methylation analysis, bisulfite sequencing . | ||||
|---|---|---|---|---|---|---|
| 84CYCLOPHILINfwd | GCTCGTGCCGTTTTGCA | BCL2 A | 203bcl2ctp84075f | TTTTGGGAGGTGGTTGTAGTTAGTG | RFP2 | |
| 85CYCLOPHILINrev | GCAAACAGCTCAAAGGAGACG | CTCCGGGAGGCGGCCGTAGCCAGCG | 303rfp2ctp38f | AAATAATATGTTGGTGATGTGATAG | ||
| 55PGKfwd | AAGTGAAGCTCGGAAAGCTTCTAT | 204bcl2ctp84129r | TTTCTCCTCCTCCTAATCCTACACA | AAATAACATGTTGGTGATGTGATAG | ||
| 56PGKrev | TGGGAAAAGATGCTTCTGGG | TTTCTCCTCCTCCTGGTCCTGCGCG | 236rfp2ctp425r | CTATAACCTAAAACCCACACCC | ||
| 11LAMIN_B1e7fwd | CTGGAAATGTTTGCATCGAAGA | BCL2 B | 263bcl2ctp85359F | TTTTGGGAAGGATGGTGTATGTT | CTGTAACCTAAGGCCCACACCC | |
| 12LAMIN_B1e8rev | GCCTCCCATTGGTTGATCC | TCTGGGAAGGATGGCGCACGCTG | 232rfp2cpgctp172f | GGGAGTTGTTTAGGTTAGTAGG | ||
| 13LAMIN_B1e1fwd | GCTGCTCCTCAACTATGCTAAGAA | 264bcl2ctp85461R | TCCCACTCATAACCCCTCTACAAC | GGGAGTTGCCCAGGTCAGCAGG | ||
| 14LAMIN_B1e2rev | TCTTTCGAATTCAGTGCTGCTTC | TCCCACTCGTAGCCCCTCTGCGAC | 236rfp2cpgctp425r | CTATAACCTAAAACCCACACCC | ||
| 89BCMSUN_e1fwd | CCTGATCTCATCAATCTAGCG | KPNA3 C | 184kpna3ctp86f | GGAGTTGTGTGTAGTTATGGTTGAGAA | CTGTAACCTAAGGCCCACACCC | |
| 91BCMSUN_e4rev | GAATTGGTTCTCTGCTACAGGTCA | GGAGCCGCGCGCAGCCATGGCCGAGAA | 235rfp2ctp404f | GGGTGTGGGTTTTAGGTTATAG | ||
| 7BCMSe1fwd | TGCCGTTCTCCAGCGC | 202kpna3ctp182rev | AACACACTCACTTCCACATCACAAC | GGGTGTGGGCCTTAGGTTACAG | ||
| 8BCMSe2rev | CCTCAGGTGTCCTCATCTGGTAA | AGCACACTCACTTCCACATCGCGGC | 238rfp2ctp737r | AATTTACCTCTATCTCCTACAAAC | ||
| 73KPNA3e2fwd | GCAGCCGCAGGAGTAGCC | KPNA3 D | 267kpna3ctp62296F | GTTTAGTGAGAATGAGGAGAATGTTGG | AGTTTACCTCTGTCTCCTACAAGC | |
| 74KPNA3e3rev | TTCCACTGTCACTTCATTTCTATGTCTT | GCCTAGTGAGAACGAGGAGAATGCCGG | BCMS | |||
| BCL-2-612F | GGCTGGGATGCCTTTGTG | 268kkpna3ctp62368R | CCAACAACATAAACACAACACACAAA | 224BCMSctp6594f | GAGTTGTGGAGTAAGAATTGATAG | |
| BCL-2-675R | GCCAGGAGAAATCAAACAGAGG | CCAGCAACATGAGCGCGGCGCGCAAA | GAGCTGTGGAGTAAGAATTGATAG | |||
| 71RB1e1fwd | TCTCGTCAGGCTTGAGTTTGAAG | RFP2 E | 195rfp2ctp95fwd | TGGAGGTTGGAGATAAGTATTTGTTGT | 229BCMSctp7123r | TTCCCAAACCCTAACTACCCT |
| 72RB1e2rev | ATAACCTCCCAATACTCCATCCAC | CGGAGGTCGGAGATAAGTACCCGCCGC | TTCCCAAGCCCTGGCTGCCCT | |||
| RFP2e1F | AAATACATCAGCTTGTAGGAGAC | 196rfp2ctp245rev | CAATACCCCACTAATTTCTACCACACTA | 228BCMSctp7103f | GGGTAGTTAGGGTTTGGGAAA | |
| 70RFP2e2rev | CCGTGGATCATCAAACAGACTACA | CAGTGCCCCGCTGGTTTCTGCCGCGCTG | GGGCAGCCAGGGCTTGGGAAA | |||
| 222RFP2e2F | ACGGATGGCCTTTAACATTGC | RFP2 F | 301rfp2ctp399F | TGTTTGAGTTTTAGTTTGGTAGTGTGGTA | 230BCMSctp7866r | AATACCTAATCTCATCAATCTAAC |
| 223RFP2e2R | CTAAACTCCTGCATCTGTTGCAGA | CGTCTGAGCTCCAGTCCGGCAGCGCGGCA | GATGCCTGATCTCATCAATCTAGC | |||
| 15CHC1Le7fwd | GACCATAGCATGTGGGCAGAT | 302rfp2ctp540R | ACAAAACCAAAAAAACTCCAAACAA | 269BCMSctp7843f | GTTAGATTGATGAGATTAGGTATTA | |
| 16CHC1Le8rev | GCCCGTTTCCGTTGTAACC | GCGGGGCCAGGAAGGCTCCGGACGG | GCTAGATTGATGAGATCAGGCATCA | |||
| 51BCMSUN_e5F | AATTCTTACCTTTCTTACCTATTTGATGAAG | BCMS G | 207BCMSctp7218f | TTGGAGGGATTTATGATTTGGG | 231BCMSctp7969r | CAACTATAAAAACAACCCAATTCC |
| 52BCMSUN_e7R | GAGGTACAGTAATTATCCACCCTTGG | CCGGAGGGACCCATGACTCCGGG | CAGCTGTGGAGGCAGCCCGGTTCC | |||
| 287BCMSUN_eB_F | GCTCCGATCCCTGCGC | 208BCMSctp7337r | CATTTCCAAACCAAACCACCTTT | 270BCMSctp7945f | GAATTGGGTTGTTTTTATAGTTGTT | |
| 91BCMSUN_e4R | GAATTGGTTCTCTGCTACAGGTCA | CATTTCCGAACCGGGCCGCCTTT | GAACGCGGGCTGCCTCCACAGCTGTC | |||
| 289BCMSUN_eB_F | ATCCCTGCGCGGGCT | BCMS H | 129BCMSctp7861f | GGTATTATTTGAAAATGGTGTTGAAAGTGT | 273BCMSctp8920r | AAAAACTACCCATTCCTATCTCAA |
| 91 BCMSUN_e4R | GAATTGGTTCTCTGCTACAGGTCA | GGCATCACTCGAAAATGGCGCCGAAAGCGC | AAAAACTGCCCATTCCTATCTCAA | |||
| 130BCMSctp7932r | AAACAACCCAATTCCCAACATTCCCT | 276BCMSctp8472f | GATTAGGAAATTTAGGTTTAGAGG | |||
| AGGCAGCCCGGTTCCCGGCATTCCCTGGG | GACCAGGAAACCTAGGCTCAGAGAC | |||||
| BCMSUN J | 252BCMSUNctp117567F | TTGGATTGAGTTGATAGTGTTGGATT | 274BCMSctp8845r | TTTACTAAATTCAATTCACTTATCAC | ||
| CCGGACCGAGTCGACAGCGTCGGACT | TTTGCTGGATTCAGTTCACTTATCAC | |||||
| 254BCMSUNctp117635R | TCCTCCTTCCCCAAAACACCA | |||||
| TCCTCCTTCCCCAGAGCGCCG | ||||||
| BCMSUN K | 257BCMSUNctp121552F | GGTTTGTGTAGATTTGGAAGAGGTG | ||||
| GGCCTGCGCAGACTTGGAAGAGGCG | ||||||
| 258BCMSUNctp121632R | AATACCTCCCCTTAACTAAAAACATCTTT | |||||
| GATGCCTCCCCTTAACTGAAAACGTCTTT | ||||||
| Expression analysis . | Methylation analysis, real-time polymerase chain reaction . | Methylation analysis, bisulfite sequencing . | ||||
|---|---|---|---|---|---|---|
| 84CYCLOPHILINfwd | GCTCGTGCCGTTTTGCA | BCL2 A | 203bcl2ctp84075f | TTTTGGGAGGTGGTTGTAGTTAGTG | RFP2 | |
| 85CYCLOPHILINrev | GCAAACAGCTCAAAGGAGACG | CTCCGGGAGGCGGCCGTAGCCAGCG | 303rfp2ctp38f | AAATAATATGTTGGTGATGTGATAG | ||
| 55PGKfwd | AAGTGAAGCTCGGAAAGCTTCTAT | 204bcl2ctp84129r | TTTCTCCTCCTCCTAATCCTACACA | AAATAACATGTTGGTGATGTGATAG | ||
| 56PGKrev | TGGGAAAAGATGCTTCTGGG | TTTCTCCTCCTCCTGGTCCTGCGCG | 236rfp2ctp425r | CTATAACCTAAAACCCACACCC | ||
| 11LAMIN_B1e7fwd | CTGGAAATGTTTGCATCGAAGA | BCL2 B | 263bcl2ctp85359F | TTTTGGGAAGGATGGTGTATGTT | CTGTAACCTAAGGCCCACACCC | |
| 12LAMIN_B1e8rev | GCCTCCCATTGGTTGATCC | TCTGGGAAGGATGGCGCACGCTG | 232rfp2cpgctp172f | GGGAGTTGTTTAGGTTAGTAGG | ||
| 13LAMIN_B1e1fwd | GCTGCTCCTCAACTATGCTAAGAA | 264bcl2ctp85461R | TCCCACTCATAACCCCTCTACAAC | GGGAGTTGCCCAGGTCAGCAGG | ||
| 14LAMIN_B1e2rev | TCTTTCGAATTCAGTGCTGCTTC | TCCCACTCGTAGCCCCTCTGCGAC | 236rfp2cpgctp425r | CTATAACCTAAAACCCACACCC | ||
| 89BCMSUN_e1fwd | CCTGATCTCATCAATCTAGCG | KPNA3 C | 184kpna3ctp86f | GGAGTTGTGTGTAGTTATGGTTGAGAA | CTGTAACCTAAGGCCCACACCC | |
| 91BCMSUN_e4rev | GAATTGGTTCTCTGCTACAGGTCA | GGAGCCGCGCGCAGCCATGGCCGAGAA | 235rfp2ctp404f | GGGTGTGGGTTTTAGGTTATAG | ||
| 7BCMSe1fwd | TGCCGTTCTCCAGCGC | 202kpna3ctp182rev | AACACACTCACTTCCACATCACAAC | GGGTGTGGGCCTTAGGTTACAG | ||
| 8BCMSe2rev | CCTCAGGTGTCCTCATCTGGTAA | AGCACACTCACTTCCACATCGCGGC | 238rfp2ctp737r | AATTTACCTCTATCTCCTACAAAC | ||
| 73KPNA3e2fwd | GCAGCCGCAGGAGTAGCC | KPNA3 D | 267kpna3ctp62296F | GTTTAGTGAGAATGAGGAGAATGTTGG | AGTTTACCTCTGTCTCCTACAAGC | |
| 74KPNA3e3rev | TTCCACTGTCACTTCATTTCTATGTCTT | GCCTAGTGAGAACGAGGAGAATGCCGG | BCMS | |||
| BCL-2-612F | GGCTGGGATGCCTTTGTG | 268kkpna3ctp62368R | CCAACAACATAAACACAACACACAAA | 224BCMSctp6594f | GAGTTGTGGAGTAAGAATTGATAG | |
| BCL-2-675R | GCCAGGAGAAATCAAACAGAGG | CCAGCAACATGAGCGCGGCGCGCAAA | GAGCTGTGGAGTAAGAATTGATAG | |||
| 71RB1e1fwd | TCTCGTCAGGCTTGAGTTTGAAG | RFP2 E | 195rfp2ctp95fwd | TGGAGGTTGGAGATAAGTATTTGTTGT | 229BCMSctp7123r | TTCCCAAACCCTAACTACCCT |
| 72RB1e2rev | ATAACCTCCCAATACTCCATCCAC | CGGAGGTCGGAGATAAGTACCCGCCGC | TTCCCAAGCCCTGGCTGCCCT | |||
| RFP2e1F | AAATACATCAGCTTGTAGGAGAC | 196rfp2ctp245rev | CAATACCCCACTAATTTCTACCACACTA | 228BCMSctp7103f | GGGTAGTTAGGGTTTGGGAAA | |
| 70RFP2e2rev | CCGTGGATCATCAAACAGACTACA | CAGTGCCCCGCTGGTTTCTGCCGCGCTG | GGGCAGCCAGGGCTTGGGAAA | |||
| 222RFP2e2F | ACGGATGGCCTTTAACATTGC | RFP2 F | 301rfp2ctp399F | TGTTTGAGTTTTAGTTTGGTAGTGTGGTA | 230BCMSctp7866r | AATACCTAATCTCATCAATCTAAC |
| 223RFP2e2R | CTAAACTCCTGCATCTGTTGCAGA | CGTCTGAGCTCCAGTCCGGCAGCGCGGCA | GATGCCTGATCTCATCAATCTAGC | |||
| 15CHC1Le7fwd | GACCATAGCATGTGGGCAGAT | 302rfp2ctp540R | ACAAAACCAAAAAAACTCCAAACAA | 269BCMSctp7843f | GTTAGATTGATGAGATTAGGTATTA | |
| 16CHC1Le8rev | GCCCGTTTCCGTTGTAACC | GCGGGGCCAGGAAGGCTCCGGACGG | GCTAGATTGATGAGATCAGGCATCA | |||
| 51BCMSUN_e5F | AATTCTTACCTTTCTTACCTATTTGATGAAG | BCMS G | 207BCMSctp7218f | TTGGAGGGATTTATGATTTGGG | 231BCMSctp7969r | CAACTATAAAAACAACCCAATTCC |
| 52BCMSUN_e7R | GAGGTACAGTAATTATCCACCCTTGG | CCGGAGGGACCCATGACTCCGGG | CAGCTGTGGAGGCAGCCCGGTTCC | |||
| 287BCMSUN_eB_F | GCTCCGATCCCTGCGC | 208BCMSctp7337r | CATTTCCAAACCAAACCACCTTT | 270BCMSctp7945f | GAATTGGGTTGTTTTTATAGTTGTT | |
| 91BCMSUN_e4R | GAATTGGTTCTCTGCTACAGGTCA | CATTTCCGAACCGGGCCGCCTTT | GAACGCGGGCTGCCTCCACAGCTGTC | |||
| 289BCMSUN_eB_F | ATCCCTGCGCGGGCT | BCMS H | 129BCMSctp7861f | GGTATTATTTGAAAATGGTGTTGAAAGTGT | 273BCMSctp8920r | AAAAACTACCCATTCCTATCTCAA |
| 91 BCMSUN_e4R | GAATTGGTTCTCTGCTACAGGTCA | GGCATCACTCGAAAATGGCGCCGAAAGCGC | AAAAACTGCCCATTCCTATCTCAA | |||
| 130BCMSctp7932r | AAACAACCCAATTCCCAACATTCCCT | 276BCMSctp8472f | GATTAGGAAATTTAGGTTTAGAGG | |||
| AGGCAGCCCGGTTCCCGGCATTCCCTGGG | GACCAGGAAACCTAGGCTCAGAGAC | |||||
| BCMSUN J | 252BCMSUNctp117567F | TTGGATTGAGTTGATAGTGTTGGATT | 274BCMSctp8845r | TTTACTAAATTCAATTCACTTATCAC | ||
| CCGGACCGAGTCGACAGCGTCGGACT | TTTGCTGGATTCAGTTCACTTATCAC | |||||
| 254BCMSUNctp117635R | TCCTCCTTCCCCAAAACACCA | |||||
| TCCTCCTTCCCCAGAGCGCCG | ||||||
| BCMSUN K | 257BCMSUNctp121552F | GGTTTGTGTAGATTTGGAAGAGGTG | ||||
| GGCCTGCGCAGACTTGGAAGAGGCG | ||||||
| 258BCMSUNctp121632R | AATACCTCCCCTTAACTAAAAACATCTTT | |||||
| GATGCCTCCCCTTAACTGAAAACGTCTTT | ||||||
The primers used and the corresponding nonconverted sequence are shown in methylation analyses by real-time polymerase chain reaction and methylation analyses by bisulfite sequencing.