Cells staining positive (%) for expression of lineage-specific and differentiation markers before and after passage in NOD/SCID mice
| Patient . | MHC . | B-lineage . | T-lineage . | Monomyeloid . | Myeloid . | Progenitor . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HLA-DR . | CD19 . | CD10 . | CD22 . | sIg . | CD2 . | CD3 . | CD4 . | CD7 . | CD8 . | CD14 . | CD11b . | CD13 . | CD33 . | CD34 . | |
| 2P | 81 | 85 | 72 | 6 | 7 | 6 | 2 | 5 | < 1 | 5 | 2 | 6 | 2 | ||
| 2M | 95 | 93 | 95 | 89 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 86 | |
| 3P | 98 | 94 | 0 | 0 | 0 | 1 | 30 | 0 | |||||||
| 3M | 90 | 91 | 1 | 92 | 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 1 | 2 | 29 | |
| 4P | 99 | 99 | 99 | 99 | 1 | < 1 | < 1 | 2 | < 1 | < 1 | < 1 | < 1 | < 1 | 44 | |
| 4M | 94 | 94 | 96 | 94 | 2 | < 1 | < 1 | < 1 | < 1 | < 1 | 8 | < 1 | < 1 | 60 | |
| 5P | 92 | 95 | 92 | 94 | 1 | < 1 | 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 1 | 95 |
| 5M | 88 | 86 | 88 | 89 | |||||||||||
| 7P | 85 | 76 | 68 | 16 | 13 | 10 | 9 | 3 | 74 | 52 | 50 | < 1 | |||
| 7M | 94 | 91 | 86 | 96 | 1 | 1 | < 1 | < 1 | < 1 | < 1 | 62 | < 1 | 36 | < 1 | |
| 8P | 7 | < 1 | < 1 | 1 | < 1 | 98 | 98 | 29 | 97 | 93 | < 1 | < 1 | 88 | < 1 | < 1 |
| 8M | < 1 | < 1 | < 1 | < 1 | < 1 | 96 | 98 | 2 | 96 | 97 | < 1 | < 1 | 93 | < 1 | < 1 |
| 10P | 86 | 91 | 87 | 90 | 2 | 6 | 6 | 3 | 3 | < 1 | 3 | < 1 | < 1 | 87 | |
| 10M | 98 | 98 | 98 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 97 | ||
| 11P | 94 | 66 | 69 | < 1 | 2 | 2 | 2 | 1 | < 1 | < 1 | < 1 | 1 | < 1 | < 1 | |
| 11M | 96 | 94 | 96 | 83 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 1 | < 1 | < 1 | 40 |
| 14P | 97 | 96 | 69 | < 1 | < 1 | < 1 | < 1 | 4 | < 1 | 1 | 2 | 1 | 77 | ||
| 14M | 95 | 98 | 96 | 97 | < 1 | < 1 | < 1 | < 1 | < 1 | 2 | < 1 | < 1 | < 1 | < 1 | < 1 |
| 16P | 21 | 1 | 26 | 10 | < 1 | 67 | 84 | < 1 | 11 | 2 | < 1 | 3 | |||
| 16M | < 1 | < 1 | 42 | 2 | 86 | 5 | < 1 | 91 | 91 | < 1 | < 1 | < 1 | < 1 | 28 | |
| 17P | 94 | 92 | 85 | 2 | 7 | 6 | 3 | 3 | < 1 | 13 | < 1 | 1 | 62 | ||
| 17M | 97 | 93 | 87 | 95 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 94 | |
| 18P | 92 | 94 | 91 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 95 | ||
| 18M | 97 | 98 | 97 | 97 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 98 |
| 19P | 98 | 94 | 92 | 90 | < 1 | 6 | 5 | 1 | 5 | < 1 | 1 | < 1 | < 1 | 49 | |
| 19M | 73 | 70 | 72 | 61 | < 1 | < 1 | < 1 | < 1 | 1 | < 1 | < 1 | 11 | < 1 | < 1 | 70 |
| Patient . | MHC . | B-lineage . | T-lineage . | Monomyeloid . | Myeloid . | Progenitor . | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HLA-DR . | CD19 . | CD10 . | CD22 . | sIg . | CD2 . | CD3 . | CD4 . | CD7 . | CD8 . | CD14 . | CD11b . | CD13 . | CD33 . | CD34 . | |
| 2P | 81 | 85 | 72 | 6 | 7 | 6 | 2 | 5 | < 1 | 5 | 2 | 6 | 2 | ||
| 2M | 95 | 93 | 95 | 89 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 86 | |
| 3P | 98 | 94 | 0 | 0 | 0 | 1 | 30 | 0 | |||||||
| 3M | 90 | 91 | 1 | 92 | 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 1 | 2 | 29 | |
| 4P | 99 | 99 | 99 | 99 | 1 | < 1 | < 1 | 2 | < 1 | < 1 | < 1 | < 1 | < 1 | 44 | |
| 4M | 94 | 94 | 96 | 94 | 2 | < 1 | < 1 | < 1 | < 1 | < 1 | 8 | < 1 | < 1 | 60 | |
| 5P | 92 | 95 | 92 | 94 | 1 | < 1 | 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 1 | 95 |
| 5M | 88 | 86 | 88 | 89 | |||||||||||
| 7P | 85 | 76 | 68 | 16 | 13 | 10 | 9 | 3 | 74 | 52 | 50 | < 1 | |||
| 7M | 94 | 91 | 86 | 96 | 1 | 1 | < 1 | < 1 | < 1 | < 1 | 62 | < 1 | 36 | < 1 | |
| 8P | 7 | < 1 | < 1 | 1 | < 1 | 98 | 98 | 29 | 97 | 93 | < 1 | < 1 | 88 | < 1 | < 1 |
| 8M | < 1 | < 1 | < 1 | < 1 | < 1 | 96 | 98 | 2 | 96 | 97 | < 1 | < 1 | 93 | < 1 | < 1 |
| 10P | 86 | 91 | 87 | 90 | 2 | 6 | 6 | 3 | 3 | < 1 | 3 | < 1 | < 1 | 87 | |
| 10M | 98 | 98 | 98 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 97 | ||
| 11P | 94 | 66 | 69 | < 1 | 2 | 2 | 2 | 1 | < 1 | < 1 | < 1 | 1 | < 1 | < 1 | |
| 11M | 96 | 94 | 96 | 83 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 1 | < 1 | < 1 | 40 |
| 14P | 97 | 96 | 69 | < 1 | < 1 | < 1 | < 1 | 4 | < 1 | 1 | 2 | 1 | 77 | ||
| 14M | 95 | 98 | 96 | 97 | < 1 | < 1 | < 1 | < 1 | < 1 | 2 | < 1 | < 1 | < 1 | < 1 | < 1 |
| 16P | 21 | 1 | 26 | 10 | < 1 | 67 | 84 | < 1 | 11 | 2 | < 1 | 3 | |||
| 16M | < 1 | < 1 | 42 | 2 | 86 | 5 | < 1 | 91 | 91 | < 1 | < 1 | < 1 | < 1 | 28 | |
| 17P | 94 | 92 | 85 | 2 | 7 | 6 | 3 | 3 | < 1 | 13 | < 1 | 1 | 62 | ||
| 17M | 97 | 93 | 87 | 95 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 94 | |
| 18P | 92 | 94 | 91 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 95 | ||
| 18M | 97 | 98 | 97 | 97 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | < 1 | 98 |
| 19P | 98 | 94 | 92 | 90 | < 1 | 6 | 5 | 1 | 5 | < 1 | 1 | < 1 | < 1 | 49 | |
| 19M | 73 | 70 | 72 | 61 | < 1 | < 1 | < 1 | < 1 | 1 | < 1 | < 1 | 11 | < 1 | < 1 | 70 |
MHC indicates major histocompatibility complex; P, patient biopsy sample; M, cells after passage through NOD/SCID mice.