Dimensions and hemodynamic parameters of nonirradiated and irradiated BM microvessels
| Parameters . | Normal BM . | Irradiated BM . | P . |
|---|---|---|---|
| Diameter (μm) | 53.4 ± 6.5 (19-114) | 35.2 ± 4.4 (13-93) | .024 |
| Vrbc(μm/s) | 398.0 ± 72.3 (95-1385) | 156.7 ± 35.5 (28-606) | .0037 |
| Vblood(μm/s) | 387.0 ± 84.0 (92-1766) | 162.0 ± 35.0 (30-570) | .014 |
| WSR (s−1) | 65.7 ± 11.4 (9-283) | 36.8 ± 5.7 (9-125) | .026 |
| WSS (dyn/cm2) | 1.3 ± 0.1 (0.1-2.2) | 0.9 ± 0.1 (0.2-3.1) | .049 |
| Parameters . | Normal BM . | Irradiated BM . | P . |
|---|---|---|---|
| Diameter (μm) | 53.4 ± 6.5 (19-114) | 35.2 ± 4.4 (13-93) | .024 |
| Vrbc(μm/s) | 398.0 ± 72.3 (95-1385) | 156.7 ± 35.5 (28-606) | .0037 |
| Vblood(μm/s) | 387.0 ± 84.0 (92-1766) | 162.0 ± 35.0 (30-570) | .014 |
| WSR (s−1) | 65.7 ± 11.4 (9-283) | 36.8 ± 5.7 (9-125) | .026 |
| WSS (dyn/cm2) | 1.3 ± 0.1 (0.1-2.2) | 0.9 ± 0.1 (0.2-3.1) | .049 |
To visualize blood flow, murine red blood cells were fluorescence labeled ex vivo and injected into the carotid artery of anesthetized mice 48 hours after TBI (9 Gy). Fluorescent cells were recorded during their passage through the skull BM, and their velocity and luminal diameters were determined by off-line video analysis using a PC-based image analysis system.60 Data are presented as mean ± SEM. Ranges are given in parentheses.
Vrbc indicates red blood cell velocity; Vblood, mean blood flow velocity; WSR, wall shear rate; WSS, wall shear stress.