Primers used for SSCP analysis
| . | . | Primer sequence 5′ 3′ . | Temperature (°C) . | MgCl2 (mM) . | Size of product (bp) . |
|---|---|---|---|---|---|
| Exon 1 | Forward | TCA-GTC-GGT-CAG-CGC-GGA-GCC-A | 57 | 1 | 228 |
| Reverse | ACT-TTT-CTC-CGC-CCG-GGC-CCA-CT | ||||
| Exon 2 | Forward | ATT-TGG-CTC-CTG-ATT-TCG-GGC-CGT | 57 | 1 | 163 |
| Reverse | CAA-CAA-CGT-ACC-CGC-GCG-CGC-C | ||||
| Exon 3 | Forward | TTG-CAT-AAT-TTA-TGT-AAT-GTC-CTG-CT | 55 | 3 | 243 |
| Reverse | GTA-AAT-TAC-CAG-ATA-ATC-TCT-AAC-AAA | ||||
| Exon 4 | Forward | CAA-TTA-AAA-ACA-TCC-TTT-TCT-GTG-T | 55 | 3 | 189 |
| Reverse | TAA-AGG-GAC-CAT-GCT-CAA-TAA-AGC-C | ||||
| Exon 5 | Forward | TCA-AAA-TTA-AAA-TAG-GTA-TTT-CAT-GTA-T | 55 | 2 | 208 |
| Reverse | AAA-CAT-CAC-AAA-ACA-CAG-CCT-GAG-A | ||||
| Exon 6 | Forward | ACT-TTT-TGA-CCC-CAA-ATT-ATG-A | 55 | 3.5 | 276 |
| Reverse | AAG-AGC-CAA-GAC-CCT-GTT-TTT-A |
| . | . | Primer sequence 5′ 3′ . | Temperature (°C) . | MgCl2 (mM) . | Size of product (bp) . |
|---|---|---|---|---|---|
| Exon 1 | Forward | TCA-GTC-GGT-CAG-CGC-GGA-GCC-A | 57 | 1 | 228 |
| Reverse | ACT-TTT-CTC-CGC-CCG-GGC-CCA-CT | ||||
| Exon 2 | Forward | ATT-TGG-CTC-CTG-ATT-TCG-GGC-CGT | 57 | 1 | 163 |
| Reverse | CAA-CAA-CGT-ACC-CGC-GCG-CGC-C | ||||
| Exon 3 | Forward | TTG-CAT-AAT-TTA-TGT-AAT-GTC-CTG-CT | 55 | 3 | 243 |
| Reverse | GTA-AAT-TAC-CAG-ATA-ATC-TCT-AAC-AAA | ||||
| Exon 4 | Forward | CAA-TTA-AAA-ACA-TCC-TTT-TCT-GTG-T | 55 | 3 | 189 |
| Reverse | TAA-AGG-GAC-CAT-GCT-CAA-TAA-AGC-C | ||||
| Exon 5 | Forward | TCA-AAA-TTA-AAA-TAG-GTA-TTT-CAT-GTA-T | 55 | 2 | 208 |
| Reverse | AAA-CAT-CAC-AAA-ACA-CAG-CCT-GAG-A | ||||
| Exon 6 | Forward | ACT-TTT-TGA-CCC-CAA-ATT-ATG-A | 55 | 3.5 | 276 |
| Reverse | AAG-AGC-CAA-GAC-CCT-GTT-TTT-A |