Oxidative resistance, conjugated dienes, thiobarbituric acid—reactive substances, total lipid hydroperoxide, and α-tocopherol content of low-density lipoprotein from normal plasma treated with heme or hemoglobin
| Parameter . | Plasma . | Plasma + heme (80 μM) . | Plasma + ferroHb (20 μM) . | Plasma + metHb (20 μM) . | Plasma + metHb + Hp (20 μM) . | Plasma + cyanometHb (20 μM) . |
|---|---|---|---|---|---|---|
| ΔT at Vmax, s | 3130 ± 272 | 150 ± 118 | 3190 ± 346 | 300 ± 189 | 3721 ± 762 | 3480 ± 722 |
| Conj dienes, OD at 234 nm | 0.175 ± 0.011 | 0.483 ± 0.099 | 0.170 ± 0.05 | 0.321 ± 0.026 | 0.189 ± 0.027 | 0.166 ± 0.005 |
| TBARSs, nmol/mg LDL | 0.32 ± 0.09 | 14.22 ± 1.46 | 0.34 ± 0.21 | 6.65 ± 0.52 | 0.21 ± 0.12 | 0.31 ± 0.14 |
| Total LOOHs, nmol/mg LDL | 5.34 ± 2.28 | 142.82 ± 25.93 | 7.50 ± 4.49 | 101.5 ± 35.02 | 5.04 ± 1.29 | 4.72 ± 3.17 |
| α-tocopherol, mol/mol ApoB-100 | 7.71 ± 2.26 | 0.80 ± 0.86 | 7.59 ± 0.93 | 0.71 ± 0.78 | 6.88 ± 0.39 | 7.29 ± 1.09 |
| Parameter . | Plasma . | Plasma + heme (80 μM) . | Plasma + ferroHb (20 μM) . | Plasma + metHb (20 μM) . | Plasma + metHb + Hp (20 μM) . | Plasma + cyanometHb (20 μM) . |
|---|---|---|---|---|---|---|
| ΔT at Vmax, s | 3130 ± 272 | 150 ± 118 | 3190 ± 346 | 300 ± 189 | 3721 ± 762 | 3480 ± 722 |
| Conj dienes, OD at 234 nm | 0.175 ± 0.011 | 0.483 ± 0.099 | 0.170 ± 0.05 | 0.321 ± 0.026 | 0.189 ± 0.027 | 0.166 ± 0.005 |
| TBARSs, nmol/mg LDL | 0.32 ± 0.09 | 14.22 ± 1.46 | 0.34 ± 0.21 | 6.65 ± 0.52 | 0.21 ± 0.12 | 0.31 ± 0.14 |
| Total LOOHs, nmol/mg LDL | 5.34 ± 2.28 | 142.82 ± 25.93 | 7.50 ± 4.49 | 101.5 ± 35.02 | 5.04 ± 1.29 | 4.72 ± 3.17 |
| α-tocopherol, mol/mol ApoB-100 | 7.71 ± 2.26 | 0.80 ± 0.86 | 7.59 ± 0.93 | 0.71 ± 0.78 | 6.88 ± 0.39 | 7.29 ± 1.09 |
Hp indicates haptoglobin. Other abbreviations are explained in Table 1.