mRNA levels of fibrinogen γ-chain and fibrinogen antigen levels
| Cell line . | Concentration of RNA (μg/mL) . | GAPDH mRNA (× 105 copy) . | γ-Chain mRNA (× 105 copy) . | Relative γ-chain mRNA (× 105 copy)* . | Fibrinogen concentration in cell lysates (μg/mL) . | Fibrinogen concentration in media (μg/mL) . |
|---|---|---|---|---|---|---|
| γ379-1 | 0.55 | 57.0 | 6.1 | 5.4 | 0.015 | ND |
| γ384-33 | 1.1 | 12.0 | 11.0 | 48 | 0.045 | ND |
| γ385-24 | 1.2 | 12.0 | 17.0 | 71 | 0.04 | ND |
| γ386-20 | 0.58 | 48.0 | 7.5 | 7.8 | 0.036 | ND |
| γ386-37 | 0.32 | 64.0 | 4.4 | 3.4 | 0.058 | ND |
| γ386-39 | 0.41 | 54.0 | 8.2 | 7.7 | 0.032 | ND |
| γ387-5 | 1.3 | 76.0 | 11.0 | 7.3 | 0.81 | 0.62 |
| γ390-16 | 0.69 | 98.0 | 5.6 | 2.9 | 0.67 | 0.62 |
| γ395-2 | 0.97 | 82.0 | 23.0 | 14 | 3.2 | 1.7 |
| γ401-25 | 1.5 | 12.0 | 10.0 | 42 | 1 | 2.1 |
| γ406-7 | 1.2 | 71.0 | 58.0 | 41 | 3.2 | 3.7 |
| γ411-31 | 0.91 | 49.0 | 9.0 | 9.2 | 0.62 | 2.2 |
| Cell line . | Concentration of RNA (μg/mL) . | GAPDH mRNA (× 105 copy) . | γ-Chain mRNA (× 105 copy) . | Relative γ-chain mRNA (× 105 copy)* . | Fibrinogen concentration in cell lysates (μg/mL) . | Fibrinogen concentration in media (μg/mL) . |
|---|---|---|---|---|---|---|
| γ379-1 | 0.55 | 57.0 | 6.1 | 5.4 | 0.015 | ND |
| γ384-33 | 1.1 | 12.0 | 11.0 | 48 | 0.045 | ND |
| γ385-24 | 1.2 | 12.0 | 17.0 | 71 | 0.04 | ND |
| γ386-20 | 0.58 | 48.0 | 7.5 | 7.8 | 0.036 | ND |
| γ386-37 | 0.32 | 64.0 | 4.4 | 3.4 | 0.058 | ND |
| γ386-39 | 0.41 | 54.0 | 8.2 | 7.7 | 0.032 | ND |
| γ387-5 | 1.3 | 76.0 | 11.0 | 7.3 | 0.81 | 0.62 |
| γ390-16 | 0.69 | 98.0 | 5.6 | 2.9 | 0.67 | 0.62 |
| γ395-2 | 0.97 | 82.0 | 23.0 | 14 | 3.2 | 1.7 |
| γ401-25 | 1.5 | 12.0 | 10.0 | 42 | 1 | 2.1 |
| γ406-7 | 1.2 | 71.0 | 58.0 | 41 | 3.2 | 3.7 |
| γ411-31 | 0.91 | 49.0 | 9.0 | 9.2 | 0.62 | 2.2 |
γ-Chain mRNA relative to GAPDH mRNA level, as described in “Materials and methods.”