Table 3. Effects of 558 loop mutations... | American Society of Hematology

Table 3.

Effects of 558 loop mutations on the FVIIIa-dependent fluorescence anisotropy of Fl-FFR-IXa

FVIII	Fl-FFR-IXa alone^3-150		Fl-FFR-IXa and FX^3-151
FVIII	Anisotropy ± sd	Δr	Anisotropy ± sd	Δr_FX
No FVIIIa	0.210 ± 0.009	–	0.215 ± 0.008	–
WT	0.243 ± 0.003	0.033	0.287 ± 0.003	0.072
Ser558Phe	0.257 ± 0.004	0.047	0.276 ± 0.001	0.061
Val559Ala	0.231 ± 0.005	0.021	0.253 ± 0.010	0.038
Asp560Ala	0.258 ± 0.006	0.048	0.252 ± 0.003	0.037
Arg562Ala	0.274 ± 0.005	0.064	0.287 ± 0.001	0.072
Gln565Arg	0.254 ± 0.002	0.044	0.262 ± 0.013	0.047

FVIII	Fl-FFR-IXa alone^3-150		Fl-FFR-IXa and FX^3-151
FVIII	Anisotropy ± sd	Δr	Anisotropy ± sd	Δr_FX
No FVIIIa	0.210 ± 0.009	–	0.215 ± 0.008	–
WT	0.243 ± 0.003	0.033	0.287 ± 0.003	0.072
Ser558Phe	0.257 ± 0.004	0.047	0.276 ± 0.001	0.061
Val559Ala	0.231 ± 0.005	0.021	0.253 ± 0.010	0.038
Asp560Ala	0.258 ± 0.006	0.048	0.252 ± 0.003	0.037
Arg562Ala	0.274 ± 0.005	0.064	0.287 ± 0.001	0.072
Gln565Arg	0.254 ± 0.002	0.044	0.262 ± 0.013	0.047

F3-150

Reactions contained 50 nM Fl-FFR-FIXa, 50 μM PSPCPE vesicles, 200 nM FVIIIa form, and were run as described in “Materials and methods.” Values represent the mean of 3 separate experiments ± standard deviation (sd).

F3-151

Reactions were performed as described above and were supplemented with 500 nM FX.

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