Table 2. Indirect MAIPA with serum and... | American Society of Hematology

Table 2.

Indirect MAIPA with serum and IgG fractions, in the absence and presence of gold

Case	Serum				Purified IgG
	Native sample	Gold (μg/mL)^*			Native sample	Gold (μg/mL)
	Native sample	0.1	1	10	Native sample	0.1	1	10
Control^†	0.01^‡	0.00	0.00	0.00	0.15	0.12	0.13	0.12
2	0.43	0.34	0.36	0.30	0.49	0.41	0.23	0.39
3	1.39	1.14	1.93	1.04	0.46	0.31	0.35	0.23
4	0.38	0.28	0.39	0.23	0.34	0.19	0.27	0.17
7	0.33	0.24	0.24	0.18	0.56	0.48	0.48	0.53
8	1.12	0.95	1.02	0.97	1.74	1.40	1.45	1.32

Case	Serum				Purified IgG
	Native sample	Gold (μg/mL)^*			Native sample	Gold (μg/mL)
	Native sample	0.1	1	10	Native sample	0.1	1	10
Control^†	0.01^‡	0.00	0.00	0.00	0.15	0.12	0.13	0.12
2	0.43	0.34	0.36	0.30	0.49	0.41	0.23	0.39
3	1.39	1.14	1.93	1.04	0.46	0.31	0.35	0.23
4	0.38	0.28	0.39	0.23	0.34	0.19	0.27	0.17
7	0.33	0.24	0.24	0.18	0.56	0.48	0.48	0.53
8	1.12	0.95	1.02	0.97	1.74	1.40	1.45	1.32

Native samples are samples without gold added.

Final concentration of gold during all stages of MAIPA. For clarity, results using 100 μg/mL are not shown.

Negative control AB serum or IgG fraction from AB serum.

ELISA OD values. Results are from a representative experiment, derived from duplicate testing against platelets from 2 donors.