Table 1.

Results from patients with thrombocytopenia

CasePlatelet count (× 109/L)Direct PIFT T/C ratio*OD values
Direct MAIPAIndirect MAIPA
ControlPatientControlPatient
3.6 0.13 1.85 0.12 0.151-153 
6.8 0.09 0.23 0.01 0.10 
22.8 nt nt 0.01 0.21 
12 nt nt nt 0.01 0.21 
35 8.8 0.01 1.63 0.02 0.08 
58 3.9 0.13 1.07 0.12 0.29 
60 1.9 0.03 > 3.5 0.02 0.26 
na positive nt nt 0.23 0.60 
na positive nt nt 0.02 0.18 
10 na na nt nt 0.09 0.151-153 
     0.091-159 0.541-159 
CasePlatelet count (× 109/L)Direct PIFT T/C ratio*OD values
Direct MAIPAIndirect MAIPA
ControlPatientControlPatient
3.6 0.13 1.85 0.12 0.151-153 
6.8 0.09 0.23 0.01 0.10 
22.8 nt nt 0.01 0.21 
12 nt nt nt 0.01 0.21 
35 8.8 0.01 1.63 0.02 0.08 
58 3.9 0.13 1.07 0.12 0.29 
60 1.9 0.03 > 3.5 0.02 0.26 
na positive nt nt 0.23 0.60 
na positive nt nt 0.02 0.18 
10 na na nt nt 0.09 0.151-153 
     0.091-159 0.541-159 
*

Flow cytometry test results as a ratio of the test result (T) compared with the control (C). Samples 8 and 9 were tested by microscopy.

Results from control healthy donor platelets and patient's own platelets tested in parallel, using SW16 anti-GPV as capture mAb.

Average result from testing at least 3 cells previously incubated with the blood group AB control sera and patient's serum tested in parallel, using SW16 anti-GPV as capture mAb.

F1-153

Result not above cut-off of twice the control result.

F1-159

Autoantibodies were eluted from the patient's platelets and tested by MAIPA.

na, not available, archive samples referred to in the text; nt, not tested.

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