Comparison of the peptide-binding capacities of different CTL lines to FITC-labeled P11 and P21
| CTL line . | Cells positive for P11 (%) . | Cells positive for P21 (%) . |
|---|---|---|
| Anti-HLA-B7 | ||
| AMB7.1 | 70.2 | 2.1 |
| HNB7.1 | 97.3 | 4.4 |
| JRB7.2 | 36.8 | 12.4 |
| AFB7.2b | 21.2 | 6.5 |
| UWB7 | 90.5 | 2.5 |
| BCB7 | 80.0 | 1.5 |
| Anti-HLA-2 | ||
| ALA2.1 | 1.2 | 2.9 |
| UWA2 | 0.1 | 8.4 |
| BCA2 | 0.5 | 15.5 |
| CTL line . | Cells positive for P11 (%) . | Cells positive for P21 (%) . |
|---|---|---|
| Anti-HLA-B7 | ||
| AMB7.1 | 70.2 | 2.1 |
| HNB7.1 | 97.3 | 4.4 |
| JRB7.2 | 36.8 | 12.4 |
| AFB7.2b | 21.2 | 6.5 |
| UWB7 | 90.5 | 2.5 |
| BCB7 | 80.0 | 1.5 |
| Anti-HLA-2 | ||
| ALA2.1 | 1.2 | 2.9 |
| UWA2 | 0.1 | 8.4 |
| BCA2 | 0.5 | 15.5 |
Extent of T-cell binding by peptides P11 and P21 was compared using 6 anti-HLA-B7 and 3 anti-HLA-A2 CTL lines by flow cytometry. Anti-HLA-B7 CTLs differentially bound P11, whereas recognition of P21 was significantly low in all cases. However, P11 failed to recognize the anti-HLA-A2 CTL lines.