Summary of CD59 expressions and somatic mutations of PIG-A in 9 patients with PNH at the previous analysis and the current analysis
| Patient . | Mutation . | Previous analysis . | Current analysis . | Duration (y) . | ||||
|---|---|---|---|---|---|---|---|---|
| PB PMN . | BM Colonies/bursts DNA (AS) . | PB PMN . | BM MNC DNA (AS) . | |||||
| CD59−(%) . | mRNA (AS) . | CD59− (%) . | DNA (AS) . | |||||
| J4 | 298C to T | 50 | 11/22 | ND | 98 | 3/10 | ND | 7 |
| 273C to A | 0/10 | ND | 2/10 | ND | ||||
| J5 | 1309C del | 65 | 10/20 | ND | 97 | 9/9 | ND | 7 |
| J3 | 383A to G | 97 | 18/20 | ND | 93 | 4/10 | ND | 7 |
| J11 | 408T del | 83 | 15/20 | ND | 82 | 7/18 | ND | 7 |
| J13 | 116C to A | 87 | 18/20 | ND | 73 | 7/10 | ND | 7 |
| J19 | 987 T ins | 78 | 14/27 | 13/25 | 61 | 5/10 | 10/10 | 8 |
| 338 T to C | ND | 2/25 | 0/15 | 1/16 | ||||
| 1003 G to T | 2/27 | 4/25 | 0/10 | 0/10 | ||||
| 1028 AA del | 0/27 | 2/25 | 0/10 | 0/10 | ||||
| J12 | 936A del | 94 | 14/20 | ND | 33 | 4/16 | ND | 6 |
| 322A del | 1/12 | ND | 2/11 | ND | ||||
| J15 | Int 5 3′splice site G to A | 99 | (Major) | ND | 57 | 4/10 | ND | 7 |
| 368A ins | (Minor) | ND | 1/9 | ND | ||||
| J16 | Int 5 5′splice site T del | 98 | 5/5 | ND | 43 | 4/18 | ND | 10 |
| Patient . | Mutation . | Previous analysis . | Current analysis . | Duration (y) . | ||||
|---|---|---|---|---|---|---|---|---|
| PB PMN . | BM Colonies/bursts DNA (AS) . | PB PMN . | BM MNC DNA (AS) . | |||||
| CD59−(%) . | mRNA (AS) . | CD59− (%) . | DNA (AS) . | |||||
| J4 | 298C to T | 50 | 11/22 | ND | 98 | 3/10 | ND | 7 |
| 273C to A | 0/10 | ND | 2/10 | ND | ||||
| J5 | 1309C del | 65 | 10/20 | ND | 97 | 9/9 | ND | 7 |
| J3 | 383A to G | 97 | 18/20 | ND | 93 | 4/10 | ND | 7 |
| J11 | 408T del | 83 | 15/20 | ND | 82 | 7/18 | ND | 7 |
| J13 | 116C to A | 87 | 18/20 | ND | 73 | 7/10 | ND | 7 |
| J19 | 987 T ins | 78 | 14/27 | 13/25 | 61 | 5/10 | 10/10 | 8 |
| 338 T to C | ND | 2/25 | 0/15 | 1/16 | ||||
| 1003 G to T | 2/27 | 4/25 | 0/10 | 0/10 | ||||
| 1028 AA del | 0/27 | 2/25 | 0/10 | 0/10 | ||||
| J12 | 936A del | 94 | 14/20 | ND | 33 | 4/16 | ND | 6 |
| 322A del | 1/12 | ND | 2/11 | ND | ||||
| J15 | Int 5 3′splice site G to A | 99 | (Major) | ND | 57 | 4/10 | ND | 7 |
| 368A ins | (Minor) | ND | 1/9 | ND | ||||
| J16 | Int 5 5′splice site T del | 98 | 5/5 | ND | 43 | 4/18 | ND | 10 |
AS indicates amplified subclones; Duration, the period between the previous analysis and the current analysis; ND, not done.