IFN-γ production by T-cell lines in response to overlapping peptides of gB492-500
| No. of amino acid (position) . | gB sequences . | Cell lines . | |
|---|---|---|---|
| 8A . | 9F . | ||
| 9 (492-500) | LMWYELSKI | 480* | 240 |
| 10 (491-500) | NLMWYELSKI | 80 | 120 |
| 9 (491-499) | NLMWYELSK | 80 | 0 |
| 9 (490-498) | DNLMWYELS | 80 | 0 |
| 9 (489-497) | RDNLMWYEL | 80 | 0 |
| 10 (492-501) | LMWYELSKIN | 160 | 100 |
| 9 (493-501) | MWYELSKIN | 140 | 20 |
| 9 (494-502) | WYELSKNINP | 80 | 20 |
| 9 (495-503) | YELSKINPT | 100 | 20 |
| No. of amino acid (position) . | gB sequences . | Cell lines . | |
|---|---|---|---|
| 8A . | 9F . | ||
| 9 (492-500) | LMWYELSKI | 480* | 240 |
| 10 (491-500) | NLMWYELSKI | 80 | 120 |
| 9 (491-499) | NLMWYELSK | 80 | 0 |
| 9 (490-498) | DNLMWYELS | 80 | 0 |
| 9 (489-497) | RDNLMWYEL | 80 | 0 |
| 10 (492-501) | LMWYELSKIN | 160 | 100 |
| 9 (493-501) | MWYELSKIN | 140 | 20 |
| 9 (494-502) | WYELSKNINP | 80 | 20 |
| 9 (495-503) | YELSKINPT | 100 | 20 |
Number of spots/106 cells in the T-cell lines induced by overnight restimulation with each peptide. Background numbers of IFN-γ–producing cells were subtracted from the values for the peptide-stimulated cells.