Structural analysis of N-linked oligosaccharide chains purified from 6-19 mAb and its variants
| IgG . | Sialylated glycoforms4-150 . | Galactosylated glycoforms4-151 . | G0/(G1 + G2) . | ||||
|---|---|---|---|---|---|---|---|
| N . | A1 . | A2 . | G0 . | G1 . | G2 . | ||
| 6-19 | 90.0 | 9.0 | 1.0 | 48.4 | 40.3 | 11.3 | 0.94 |
| 6-19Q6E/K23A | 86.2 | 11.5 | 2.3 | 53.7 | 36.7 | 9.6 | 1.16 |
| 6-19N471T | 89.6 | 10.0 | 0.4 | 47.5 | 42.1 | 10.4 | 0.90 |
| A6C | 85.1 | 12.8 | 2.1 | 31.6 | 44.5 | 23.9 | 0.46 |
| P11C | 84.3 | 13.7 | 2.0 | 32.5 | 45.9 | 21.6 | 0.48 |
| Y12C | 87.4 | 10.8 | 1.8 | 33.6 | 45.3 | 21.1 | 0.51 |
| IgG . | Sialylated glycoforms4-150 . | Galactosylated glycoforms4-151 . | G0/(G1 + G2) . | ||||
|---|---|---|---|---|---|---|---|
| N . | A1 . | A2 . | G0 . | G1 . | G2 . | ||
| 6-19 | 90.0 | 9.0 | 1.0 | 48.4 | 40.3 | 11.3 | 0.94 |
| 6-19Q6E/K23A | 86.2 | 11.5 | 2.3 | 53.7 | 36.7 | 9.6 | 1.16 |
| 6-19N471T | 89.6 | 10.0 | 0.4 | 47.5 | 42.1 | 10.4 | 0.90 |
| A6C | 85.1 | 12.8 | 2.1 | 31.6 | 44.5 | 23.9 | 0.46 |
| P11C | 84.3 | 13.7 | 2.0 | 32.5 | 45.9 | 21.6 | 0.48 |
| Y12C | 87.4 | 10.8 | 1.8 | 33.6 | 45.3 | 21.1 | 0.51 |
mAb indicates monoclonal antibody; Ig, immunoglobulin.
Percent molar ratios of neutral asialo (N), acidic monosialo (A1), and acidic disialo (A2) oligosaccharides were calculated on the basis of their ultraviolet (UV) absorbance at 304 nm on diethylaminoethyl–high-performance liquid chromatography (HPLC).
Percent molar ratios of non (G0), mono (G1), and digalactosylated (G2) glycoforms in desialylated, neutral oligosaccharides were calculated on the basis of their UV absorbance at 304 nm on octadecylsilane-HPLC.