Vh residues at positions 6 and 23 in various IgG3 mAbs with or without cryoglobulin activity
| mAb . | Vh6* . | Vh23* . | Vhfamily . | Specificity . | Cryoglobulin† . |
|---|---|---|---|---|---|
| 6-19 | Q | K | J558 | IgG2a | + |
| 7B6.8 | Q | K | J558 | DNA | + |
| 1G3 | Q | K | J558 | DNA | + |
| 1G2 | Q | K | J558 | DNA | + |
| 8A4 | Q | K | J558 | Hydrogenase | + |
| AM16 | Q | K | Vgam3.8 | IgG2a | + |
| DP7 | Q | K | J558 | DNA | − |
| S57 | Q | K | J558 | DNA | − |
| 411H12 | Q | K | J558 | IgG1 | − |
| R4A12 | E | A | S107 | DNA | − |
| S106 | E | A | 7183 | DNA | − |
| 2-6D | E | T | 7183 | Nuclear antigen | − |
| 11F8 | E | T | Q52 | DNA | − |
| SH11 | E | P | VH10 | DNA | + |
| C11M | E | S | 36-60 | DNP | + |
| mAb . | Vh6* . | Vh23* . | Vhfamily . | Specificity . | Cryoglobulin† . |
|---|---|---|---|---|---|
| 6-19 | Q | K | J558 | IgG2a | + |
| 7B6.8 | Q | K | J558 | DNA | + |
| 1G3 | Q | K | J558 | DNA | + |
| 1G2 | Q | K | J558 | DNA | + |
| 8A4 | Q | K | J558 | Hydrogenase | + |
| AM16 | Q | K | Vgam3.8 | IgG2a | + |
| DP7 | Q | K | J558 | DNA | − |
| S57 | Q | K | J558 | DNA | − |
| 411H12 | Q | K | J558 | IgG1 | − |
| R4A12 | E | A | S107 | DNA | − |
| S106 | E | A | 7183 | DNA | − |
| 2-6D | E | T | 7183 | Nuclear antigen | − |
| 11F8 | E | T | Q52 | DNA | − |
| SH11 | E | P | VH10 | DNA | + |
| C11M | E | S | 36-60 | DNP | + |
mAb indicates monoclonal antibody; Q, glutamine; E, glutamic acid; K, lysine; A, alanine; T, threonine; P, proline; S, serine.
Amino acid residues at Vh positions 6 and 23. The Vh sequence of 7B6.8, 1G3,211G2,22 8A4,37 AM16,20 DP7, S57, S106,19 R4A12,23 and 11F824 mAbs were taken from the literature. The Vh sequences of 411H12, 2-6D, SH11, and C11M mAbs were determined in the present study.
Cryoglobulin activity of 6-19,17 7B6.8, 1G3,21 1G2,22 8A4,37AM16,30 2-6D,25 11F8,24SH11,26 and C11M9 mAbs has been described. Cryoglobulin activity of DP7, S54, and 411H12 mAbs was determined in the present study.