Table 1. Cryoglobulin activity and N... | American Society of Hematology

Table 1.

Cryoglobulin activity and N-linked oligosaccharide content of 6-19, 6-19Q6E/K23A, and 6-19N471T mAbs

mAb	Ig isotype	Vh6/23^*	Cryoglobulin^†	Oligosaccharide^‡
6-19	IgG3	Q/K	29	112
6-19Q6E/K23A	IgG3	E/A	14	112
6-19N471T	IgG3	Q/K	27	108
SS2F8	IgG1	Q/K	< 1	115

mAb	Ig isotype	Vh6/23^*	Cryoglobulin^†	Oligosaccharide^‡
6-19	IgG3	Q/K	29	112
6-19Q6E/K23A	IgG3	E/A	14	112
6-19N471T	IgG3	Q/K	27	108
SS2F8	IgG1	Q/K	< 1	115

mAb indicates monoclonal antibody; Ig, immunoglobulin; Q, glutamine; E, glutamic acid; K, lysine; A, alanine.

Amino acid residues at positions 6 and 23 of heavy chains.

†

Concentrations of IgG (μg/mL) in cryoprecipitates recovered following the incubation of mAb (1 mg/mL) for 48 hours at 4°C.

‡

Amounts of N-linked oligosaccharides liberated from different 6-19 mAbs (unit/mg of IgG3). One unit of oligosaccharides was designated as 1 μL of ABEE-oligosaccharide solution with the absorbance at 304 nm equals 1.0.

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