Table 2.

Normalized array hybridization signal intensities and relative levels of expression of rhesus macaque chemokine messenger RNAs in spleen

ChemokineDisease stateRatios
AIDSAcuteUIAIDS:UIAIDS:acuteUI:AIDS
BLC/BCA-1/CXCL13 0.64 0.47 0.22 2.9* 1.3 0.3 
MIG/CXCL9 0.50 0.30 0.15 3.3* 1.7 0.3 
IP-10/CXCL10 0.38 0.34 0.32 1.2 1.1 0.8 
Midkine 0.35 0.39 0.47 0.8 0.9 1.3 
6Ckine/CCL21 0.32 0.33 0.44 0.7 1.0 1.3 
MIP-3β/CCL19 0.26 0.24 0.14 1.9 1.1 0.5 
ENA-78/CXCL5 0.25 0.22 0.16 1.6 1.2 0.6 
GRO-γ/CXCL3 0.21 0.25 0.50 0.4 0.9 2.3* 
SDF-1/CXCL12 0.21 0.35 0.26 0.8 0.6 1.2 
LDGF 0.20 0.17 0.41 0.5 1.2 2.0* 
MIP-1β/CCL4 0.19 0.22 0.61 0.3 0.8 3.3* 
GRO-β/CXCL2 0.18 0.21 0.54 0.3 0.8 3.0* 
MIP-1α/CCL3 0.17 0.12 0.20 0.9 1.5 1.2 
RANTES/CCL5 0.17 0.30 0.25 0.7 0.6 1.5 
Lymphotactin/XCL1 0.14 0.16 0.38 0.4 0.9 2.6* 
MPIF-1/CCL23 0.14 0.18 0.22 0.6 0.8 1.6 
DC-CK-1/PARC/CCL18 0.13 0.13 0.17 0.8 1.0 1.3 
GRO-α/CXCL1 0.13 0.19 0.50 0.3 0.7 3.9* 
Eotaxin-2/CCL24 0.12 0.12 0.15 0.8 1.0 1.2 
MCP-1/CCL2 0.11 0.14 0.27 0.4 0.8 2.4* 
HCC-4/CCL16 0.11 0.11 0.16 0.6 0.9 1.6 
Fractalkine/CX3CL1 0.10 0.17 0.18 0.6 0.6 1.7 
MCP-4/CCL13 0.10 0.10 0.11 0.9 1.0 1.1 
HCC-1/CCL14 0.10 0.10 0.16 0.6 1.0 1.7 
MCP-2/CCL8 0.09 0.14 0.13 0.7 0.6 1.5 
IL-8/CXCL8 0.09 0.04 0.12 0.8 2.1 1.3 
MCP-3/CCL7 0.08 0.10 0.12 0.7 0.8 1.4 
MIP-3α/CCL20 0.08 0.07 0.15 0.5 1.1 1.9 
I-309/CCL1 0.08 0.04 0.13 0.6 1.8 1.7 
MIP-1δ/CCL15 0.08 0.11 0.38 0.2 0.7 5.0* 
TARC/CCL17 0.07 0.10 0.07 1.0 0.7 1.0 
Eotaxin/CCL11 0.07 0.08 0.14 0.5 0.9 2.1 
MDC/CCL22 0.04 0.10 0.16 0.3 0.4 3.8 
TECK/CCL25 0.03 0.08 0.15 0.2 0.3 5.5 
ChemokineDisease stateRatios
AIDSAcuteUIAIDS:UIAIDS:acuteUI:AIDS
BLC/BCA-1/CXCL13 0.64 0.47 0.22 2.9* 1.3 0.3 
MIG/CXCL9 0.50 0.30 0.15 3.3* 1.7 0.3 
IP-10/CXCL10 0.38 0.34 0.32 1.2 1.1 0.8 
Midkine 0.35 0.39 0.47 0.8 0.9 1.3 
6Ckine/CCL21 0.32 0.33 0.44 0.7 1.0 1.3 
MIP-3β/CCL19 0.26 0.24 0.14 1.9 1.1 0.5 
ENA-78/CXCL5 0.25 0.22 0.16 1.6 1.2 0.6 
GRO-γ/CXCL3 0.21 0.25 0.50 0.4 0.9 2.3* 
SDF-1/CXCL12 0.21 0.35 0.26 0.8 0.6 1.2 
LDGF 0.20 0.17 0.41 0.5 1.2 2.0* 
MIP-1β/CCL4 0.19 0.22 0.61 0.3 0.8 3.3* 
GRO-β/CXCL2 0.18 0.21 0.54 0.3 0.8 3.0* 
MIP-1α/CCL3 0.17 0.12 0.20 0.9 1.5 1.2 
RANTES/CCL5 0.17 0.30 0.25 0.7 0.6 1.5 
Lymphotactin/XCL1 0.14 0.16 0.38 0.4 0.9 2.6* 
MPIF-1/CCL23 0.14 0.18 0.22 0.6 0.8 1.6 
DC-CK-1/PARC/CCL18 0.13 0.13 0.17 0.8 1.0 1.3 
GRO-α/CXCL1 0.13 0.19 0.50 0.3 0.7 3.9* 
Eotaxin-2/CCL24 0.12 0.12 0.15 0.8 1.0 1.2 
MCP-1/CCL2 0.11 0.14 0.27 0.4 0.8 2.4* 
HCC-4/CCL16 0.11 0.11 0.16 0.6 0.9 1.6 
Fractalkine/CX3CL1 0.10 0.17 0.18 0.6 0.6 1.7 
MCP-4/CCL13 0.10 0.10 0.11 0.9 1.0 1.1 
HCC-1/CCL14 0.10 0.10 0.16 0.6 1.0 1.7 
MCP-2/CCL8 0.09 0.14 0.13 0.7 0.6 1.5 
IL-8/CXCL8 0.09 0.04 0.12 0.8 2.1 1.3 
MCP-3/CCL7 0.08 0.10 0.12 0.7 0.8 1.4 
MIP-3α/CCL20 0.08 0.07 0.15 0.5 1.1 1.9 
I-309/CCL1 0.08 0.04 0.13 0.6 1.8 1.7 
MIP-1δ/CCL15 0.08 0.11 0.38 0.2 0.7 5.0* 
TARC/CCL17 0.07 0.10 0.07 1.0 0.7 1.0 
Eotaxin/CCL11 0.07 0.08 0.14 0.5 0.9 2.1 
MDC/CCL22 0.04 0.10 0.16 0.3 0.4 3.8 
TECK/CCL25 0.03 0.08 0.15 0.2 0.3 5.5 

The values in the first 3 columns are normalized signal-intensity values obtained by DNA filter array hybridization. AIDS indicates acquired immunodeficiency syndrome; and UI, uninfected.

*

Ratios of normalized signal intensities ≥ 2.0 and that are derived by values of which one is ≥ 0.20. The mean ± 2 SD of the negative control hybridization targets on these filters ranged from 0.13 to 0.19, and we used a normalized signal-intensity value of 0.2 as a highly conservative cutoff value for what was considered to be at or above the level of sensitivity of the assay.

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