Flow cytometric analysis of graft-derived T cells
| Sample . | Recovered cells (× 106) . | Positive cells (%) . | |||
|---|---|---|---|---|---|
| Thy-1 . | W6/32 . | TCRαβ . | TCRγδ . | ||
| G1 | 1.8 | NS | 99.8 | 0.9 | 4.5 |
| G2 | 3.4 | NS | 99.5 | 0.8 | 28.8 |
| G3 | 1.3 | NS | 99.7 | 37.4 | 15.8 |
| G4 | 40.0 | NS | 99.9 | 95.9 | 1.0 |
| Sample . | Recovered cells (× 106) . | Positive cells (%) . | |||
|---|---|---|---|---|---|
| Thy-1 . | W6/32 . | TCRαβ . | TCRγδ . | ||
| G1 | 1.8 | NS | 99.8 | 0.9 | 4.5 |
| G2 | 3.4 | NS | 99.5 | 0.8 | 28.8 |
| G3 | 1.3 | NS | 99.7 | 37.4 | 15.8 |
| G4 | 40.0 | NS | 99.9 | 95.9 | 1.0 |
Cells were isolated from the mucosa of 1 simple bronchial graft (G1), 1 airway–LN cograft (G2), and 2 airway–LN–thymus cografts (G3 and G4) from 2 independent series (GA, 16 wk; DE, 10 wk for G1, G2, and G3; GA, 21 wk; DE, 27 wk for G4), expanded in vitro for 12 days and finally counted and analyzed by flow cytometry (see “Materials and methods”). Levels below 0.3% were considered not significant (NS).