Cografts of human fetal bronchus and autologous lymphoid tissues
| Type of cograft . | N . | DE (wk) . | Lymphoid tissue fate . | Enrichment in leukocytes . | Presence of BALT/IgA . | Impact on host survival . |
|---|---|---|---|---|---|---|
| Bronchus + liver | 3 | 11-21 | Involuted (3 of 3) | No (3 of 3) | No (3 of 3)/no (3 of 3) | No (3 of 3) |
| Bronchus + bone | 3 | 12-18 | Conserved (3 of 3) | No (3 of 3) | No (3 of 3)/no (3 of 3) | No (3 of 3) |
| Bronchus + thymus | 4 | 12-34 | Conserved (4 of 4) | No (4 of 4) | No (4 of 4)/no (4 of 4) | No (4 of 4) |
| Bronchus + dissected PLN | 4 | 8-28 | Involuted (4 of 4) | No (4 of 4) | No (4 of 4)/no (4 of 4) | No (4 of 4) |
| Bronchus + attached PLN | 16 | 8-36 | Conserved (16 of 16) | Yes (16 of 16) | Yes (12 of 16)/yes (16 of 16) | No (16 of 16) |
| Bronchus + attached PLN + thymus | 3 | 12-27 | Conserved (3 of 3) | Yes (3 of 3) | Yes (3 of 3)/yes (3 of 3) | No (3 of 3) |
| Type of cograft . | N . | DE (wk) . | Lymphoid tissue fate . | Enrichment in leukocytes . | Presence of BALT/IgA . | Impact on host survival . |
|---|---|---|---|---|---|---|
| Bronchus + liver | 3 | 11-21 | Involuted (3 of 3) | No (3 of 3) | No (3 of 3)/no (3 of 3) | No (3 of 3) |
| Bronchus + bone | 3 | 12-18 | Conserved (3 of 3) | No (3 of 3) | No (3 of 3)/no (3 of 3) | No (3 of 3) |
| Bronchus + thymus | 4 | 12-34 | Conserved (4 of 4) | No (4 of 4) | No (4 of 4)/no (4 of 4) | No (4 of 4) |
| Bronchus + dissected PLN | 4 | 8-28 | Involuted (4 of 4) | No (4 of 4) | No (4 of 4)/no (4 of 4) | No (4 of 4) |
| Bronchus + attached PLN | 16 | 8-36 | Conserved (16 of 16) | Yes (16 of 16) | Yes (12 of 16)/yes (16 of 16) | No (16 of 16) |
| Bronchus + attached PLN + thymus | 3 | 12-27 | Conserved (3 of 3) | Yes (3 of 3) | Yes (3 of 3)/yes (3 of 3) | No (3 of 3) |
Portions of fetal bronchi (total of 10 independent tissues from 16-25 wks gestation) were implanted in SCID hosts either alone (total of 16 simple bronchial grafts; DE, 8-36 wks) or with autologous lymphoid tissues (number of each type, N, indicated in table) and studied as described (see “Materials and methods”) after variable duration of engraftment (DE). The fate of lymphoid tissues was assessed morphologically. Bronchus–lymphoid tissue cografts were compared to simple bronchial grafts from the same series (same fetal tissue of origin, grafted in littermates) to detect any enrichment in human leukocytes, the presence of BALT, IgA, and impact on host survival.