Flow cytometric analysis of intracellular cytokines in PBMCs stimulated or not stimulated with HBenvAg in vitro from XLA patients or healthy controls
. | IFN-γ alone . | IL-4 alone . | IFN-γ and IL-4 . | |||
---|---|---|---|---|---|---|
No Ag . | Ag . | No Ag . | Ag . | No Ag . | Ag . | |
Patient no. | ||||||
1 | 0 | 0.54 | 0 | 0.34 | 0 | 0.07 |
2 | 0.02 | 1.95 | 0.01 | 0.33 | 0 | 0.03 |
3 | 0 | 0.25 | 0.02 | 0.18 | 0 | 0 |
4 | 0 | 0.65 | 0 | 0 | 0 | 0 |
5 | 0 | 0.83 | 0 | 0.48 | 0 | 0.10 |
6 | 0.01 | 1.18 | 0.01 | 0.65 | 0.02 | 0.8 |
Control subject no. | ||||||
1 | 0 | 1.12 | 0.02 | 0.23 | 0 | 0.12 |
2 | 0 | 0.45 | 0 | 0.15 | 0 | 0.05 |
. | IFN-γ alone . | IL-4 alone . | IFN-γ and IL-4 . | |||
---|---|---|---|---|---|---|
No Ag . | Ag . | No Ag . | Ag . | No Ag . | Ag . | |
Patient no. | ||||||
1 | 0 | 0.54 | 0 | 0.34 | 0 | 0.07 |
2 | 0.02 | 1.95 | 0.01 | 0.33 | 0 | 0.03 |
3 | 0 | 0.25 | 0.02 | 0.18 | 0 | 0 |
4 | 0 | 0.65 | 0 | 0 | 0 | 0 |
5 | 0 | 0.83 | 0 | 0.48 | 0 | 0.10 |
6 | 0.01 | 1.18 | 0.01 | 0.65 | 0.02 | 0.8 |
Control subject no. | ||||||
1 | 0 | 1.12 | 0.02 | 0.23 | 0 | 0.12 |
2 | 0 | 0.45 | 0 | 0.15 | 0 | 0.05 |
Data are expressed as the percentage of positive cells expressing the indicated substance.
IFN indicates interferon; IL, interleukin; and Ag, antigen.