Effect of treatment with cytokines on number of dendritic cells (DCs) in mouse spleens
| DC subset . | Cytokine used . | ||||||
|---|---|---|---|---|---|---|---|
| None . | ProGP-1 (20 μg/d) . | G-CSF (10 μg/d) . | muFL (10 μg/d) . | huFL (10 μg/d) . | G-CSF + muFL (10 μg/d) . | pGM-CSF (2 μg/d) . | |
| Total DCs | 1.60 | 53.4 | 3.6 | 15.0 | 38.7 | 18.8 | 19.0 |
| CD4+CD8− | 0.75 | 0.9 | 1.5 | 1.8 | 4.3 | 1.4 | 9.9 |
| CD4−CD8+ | 0.35 | 45.4 | 1.0 | 9.5 | 17.0 | 13.5 | 2.0 |
| CD4−CD8− | 0.46 | 4.3 | 0.8 | 2.1 | 16.6 | 2.6 | 6.7 |
| DC subset . | Cytokine used . | ||||||
|---|---|---|---|---|---|---|---|
| None . | ProGP-1 (20 μg/d) . | G-CSF (10 μg/d) . | muFL (10 μg/d) . | huFL (10 μg/d) . | G-CSF + muFL (10 μg/d) . | pGM-CSF (2 μg/d) . | |
| Total DCs | 1.60 | 53.4 | 3.6 | 15.0 | 38.7 | 18.8 | 19.0 |
| CD4+CD8− | 0.75 | 0.9 | 1.5 | 1.8 | 4.3 | 1.4 | 9.9 |
| CD4−CD8+ | 0.35 | 45.4 | 1.0 | 9.5 | 17.0 | 13.5 | 2.0 |
| CD4−CD8− | 0.46 | 4.3 | 0.8 | 2.1 | 16.6 | 2.6 | 6.7 |
Values are the number of DCs/per spleen × 106. Treatment was done for 10 days (d), except for that with pegylated granulocyte-macrophage colony-stimulating factor (pGM-CSF), which was done for 5 days. Values are from 1 experiment representative of at least 3 experiments using a minimum of 3 mice for each cytokine treatment and for untreated controls.
ProGP-1 indicates progenipoietin-1; G-CSF, granulocyte colony-stimulating factor; muFL, murine Flt-3 ligand; and huFL, human Flt-3 ligand.