Comparison of Ig and TCR gene rearrangement patterns based on Southern blotting
| . | Random precursor B–ALL at diagnosis* . | Relapsed precursor-B–ALL at diagnosis† . | Precursor-B–ALL at relapse . |
|---|---|---|---|
| IGH | |||
| Germline | 0 | 0 | 0 |
| R/G | 1% (1/97) | 2% (2/92) | 5% (4/85) |
| R/R | 88% (85/97) | 88% (81/92) | 85% (72/85) |
| R/D | 8% (8/97) | 7% (6/92) | 7% (6/85) |
| D/G | 2% (2/97) | 1% (1/92) | 1% (1/85) |
| D/D | 1% (1/97) | 1% (1/92) | 2% (2/85) |
| Oligoclonal | 36% (35/97) | 42% (38/92)‡ | 28% (24/85)1-153 |
| IGK-Kde | |||
| Germline | 50% (55/111) | 51% (47/92) | 49% (42/86) |
| R/G | 28% (31/111) | 21% (19/92) | 23% (20/86) |
| R/R | 23% (25/111) | 28% (26/92) | 28% (24/86) |
| Oligoclonal | Not determined | 3% (3/92) | 1% (1/86) |
| TCRG | |||
| Germline | 41% (79/192) | 38% (35/91) | 41% (37/91) |
| R/G or R/R | 59% (113/192) | 62% (56/91) | 59% (54/91) |
| TCRD | |||
| Germline | 11% (22/202) | 8% (7/91) | 12% (10/85) |
| R/G | 15% (30/202) | 11% (10/91) | 8% (7/85) |
| R/R | 23% (47/202) | 24% (22/91) | 12% (10/85)1-155 |
| R/D | 17% (35/202) | 20% (18/91) | 22% (19/85) |
| D/G | 5% (11/202) | 4% (4/91) | 5% (4/85) |
| D/D | 28% (57/202) | 33% (30/91) | 41% (35/85)1-155 |
| Oligoclonal | 21% (13/62) | 26% (24/91) | 12% (10/85)1-155 |
| . | Random precursor B–ALL at diagnosis* . | Relapsed precursor-B–ALL at diagnosis† . | Precursor-B–ALL at relapse . |
|---|---|---|---|
| IGH | |||
| Germline | 0 | 0 | 0 |
| R/G | 1% (1/97) | 2% (2/92) | 5% (4/85) |
| R/R | 88% (85/97) | 88% (81/92) | 85% (72/85) |
| R/D | 8% (8/97) | 7% (6/92) | 7% (6/85) |
| D/G | 2% (2/97) | 1% (1/92) | 1% (1/85) |
| D/D | 1% (1/97) | 1% (1/92) | 2% (2/85) |
| Oligoclonal | 36% (35/97) | 42% (38/92)‡ | 28% (24/85)1-153 |
| IGK-Kde | |||
| Germline | 50% (55/111) | 51% (47/92) | 49% (42/86) |
| R/G | 28% (31/111) | 21% (19/92) | 23% (20/86) |
| R/R | 23% (25/111) | 28% (26/92) | 28% (24/86) |
| Oligoclonal | Not determined | 3% (3/92) | 1% (1/86) |
| TCRG | |||
| Germline | 41% (79/192) | 38% (35/91) | 41% (37/91) |
| R/G or R/R | 59% (113/192) | 62% (56/91) | 59% (54/91) |
| TCRD | |||
| Germline | 11% (22/202) | 8% (7/91) | 12% (10/85) |
| R/G | 15% (30/202) | 11% (10/91) | 8% (7/85) |
| R/R | 23% (47/202) | 24% (22/91) | 12% (10/85)1-155 |
| R/D | 17% (35/202) | 20% (18/91) | 22% (19/85) |
| D/G | 5% (11/202) | 4% (4/91) | 5% (4/85) |
| D/D | 28% (57/202) | 33% (30/91) | 41% (35/85)1-155 |
| Oligoclonal | 21% (13/62) | 26% (24/91) | 12% (10/85)1-155 |
The frequencies of particular Ig/TCR gene rearrangements in the random precursor-B–ALL group at diagnosis are derived from our previous studies.6,27 46
Including one patient with phenotypic switch to AML at relapse.
The frequency of IGH oligoclonality was significantly higher in infant ALL compared with noninfant ALL (75% vs 38%, P < .05).
Not significant.
P < .05.