Morphologic properties of individual VWF molecules after 5 minutes of perfusion with 1 μg/mL VWF over collagen as determined by image analysis of the digital pictures (mean ± SD)
| Shear stress . | First experiment . | Second experiment . | ||
|---|---|---|---|---|
| 0.07 N/m2 . | 4.55 N/m2 . | 0.07 N/m2 . | 4.55 N/m2 . | |
| Number of measured molecules | 210 | 378 | 186 | 336 |
| Surface coverage, % | 4.06 | 7.92 | 4.94 | 8.04 |
| Mean area, square pixels | 253 ± 180 | 330 ± 434 | 279 ± 152 | 314 ± 440 |
| Mean perimeter, pixels | 71 ± 38 | 79 ± 60 | 74 ± 27 | 87 ± 88 |
| Mean major axis length, pixels | 22 ± 11 | 25 ± 14 | 24 ± 8 | 27 ± 16 |
| Mean major axis angle, degrees | 15 ± 43 | 10 ± 38 | 21 ± 34 | 20 ± 35 |
| Mean minor axis length, pixels | 14 ± 5 | 15 ± 9 | 15 ± 5 | 15 ± 8 |
| Mean minor axis angle, degrees | −13 ± 59 | −13 ± 63 | −28 ± 59 | −25 ± 58 |
| Mean eccentricity | 1.67 ± 0.58 | 1.62 ± 0.42 | 1.77 ± 0.59 | 1.83 ± 0.55 |
| Mean roundness | 0.66 ± 0.20 | 0.67 ± 0.19 | 0.64 ± 0.14 | 0.58 ± 0.17 |
| Mean feret diameter, pixels | 17 ± 6 | 19 ± 9 | 18 ± 5 | 19 ± 8 |
| Shear stress . | First experiment . | Second experiment . | ||
|---|---|---|---|---|
| 0.07 N/m2 . | 4.55 N/m2 . | 0.07 N/m2 . | 4.55 N/m2 . | |
| Number of measured molecules | 210 | 378 | 186 | 336 |
| Surface coverage, % | 4.06 | 7.92 | 4.94 | 8.04 |
| Mean area, square pixels | 253 ± 180 | 330 ± 434 | 279 ± 152 | 314 ± 440 |
| Mean perimeter, pixels | 71 ± 38 | 79 ± 60 | 74 ± 27 | 87 ± 88 |
| Mean major axis length, pixels | 22 ± 11 | 25 ± 14 | 24 ± 8 | 27 ± 16 |
| Mean major axis angle, degrees | 15 ± 43 | 10 ± 38 | 21 ± 34 | 20 ± 35 |
| Mean minor axis length, pixels | 14 ± 5 | 15 ± 9 | 15 ± 5 | 15 ± 8 |
| Mean minor axis angle, degrees | −13 ± 59 | −13 ± 63 | −28 ± 59 | −25 ± 58 |
| Mean eccentricity | 1.67 ± 0.58 | 1.62 ± 0.42 | 1.77 ± 0.59 | 1.83 ± 0.55 |
| Mean roundness | 0.66 ± 0.20 | 0.67 ± 0.19 | 0.64 ± 0.14 | 0.58 ± 0.17 |
| Mean feret diameter, pixels | 17 ± 6 | 19 ± 9 | 18 ± 5 | 19 ± 8 |
As all morphologic measures are performed on digitalized images, dimensions are given in pixels instead of nanometers. Theoretically on a 5 × 5-μm image sampled at 512 pixels per line and 512 pixels per row, one pixel corresponds to approximately 9.77 nm (real dimensions are, however, somewhat different because of tip spatial convolution effects).