Analyses of the human tonsillar CD34
| No. . | m/z . | % . | Proposed composition . |
|---|---|---|---|
| N-linked glycans | |||
| N-1 | 1713.1 | 1.5 | SA1Hex1HexNAc1+ Man3GlcNAc2Fuc1 |
| N-2 | 1875.3 | 1.5 | SA1Hex2HexNAc1+ Man3GlcNAc2Fuc1 |
| N-3 | 2076.9 | 36 | SA1Hex2HexNAc2+ Man3GlcNAc2Fuc1 |
| N-4 | 2222.9 | 3.5 | SA2Hex2HexNAc2+ Man3GlcNAc2Fuc1 |
| N-5 | 2281.7 | 1 | SA1Hex2HexNAc3+ Man3GlcNAc2Fuc1 |
| N-6 | 2368.0 | 30 | SA2Hex2HexNAc2+ Man3GlcNAc2Fuc1 |
| N-7 | 2442.0 | 2 | SA1Hex3HexNAc3+ Man3GlcNAc2Fuc1 |
| N-8 | 2571.3 | 1.5 | SA2Hex2HexNAc3+ Man3GlcNAc2Fuc1 |
| N-9 | 2733.2 | 6 | SA2Hex3HexNAc3+ Man3GlcNAc2Fuc1 |
| N-10 | 3025.1 | 10 | SA3Hex3HexNAc3+ Man3GlcNAc2Fuc1 |
| N-11 | 3100.2 | 1.5 | SA2Hex4HexNAc4+ Man3GlcNAc2Fuc1 |
| N-12 | 3391.2 | 3 | SA3Hex4HexNAc4+ Man3GlcNAc2Fuc1 |
| N-13 | 3682.5 | 1.5 | SA4Hex4HexNAc4+ Man3GlcNAc2Fuc1 |
| N-14 | 3758.1 | 0.5 | SA3Hex5HexNAc5+ Man3GlcNAc2Fuc1 |
| N-15 | 4049.0 | 0.5 | SA4Hex5HexNAc5+ Man3GlcNAc2Fuc1 |
| O-linked glycan alditols | |||
| O-1 | 674.8 | 53 | SA1Hex1HexNAc1 |
| O-2 | 966.4 | 10 | SA2Hex1HexNAc1 |
| O-3 | 1040.6 | 5 | SA1Hex2HexNAc2 |
| O-4 | 1186.3 | 0.5 | SA1Hex2HexNAc2Fuc1 |
| O-5 | 1333.0 | 21 | SA2Hex2HexNAc2 |
| O-6 | 1413.8 | 1 | SA2Hex2HexNAc2(SO3)1 |
| O-7 | 1698.4 | 5 | SA2Hex3HexNAc3 |
| O-8 | 1778.7 | 2 | SA2Hex3HexNAc3(SO3)1 |
| O-9 | 1925.9 | 1 | SA2Hex3HexNAc3(SO3)1Fuc1 |
| O-10 | 2062.1 | 1 | SA2Hex4HexNAc4 |
| No. . | m/z . | % . | Proposed composition . |
|---|---|---|---|
| N-linked glycans | |||
| N-1 | 1713.1 | 1.5 | SA1Hex1HexNAc1+ Man3GlcNAc2Fuc1 |
| N-2 | 1875.3 | 1.5 | SA1Hex2HexNAc1+ Man3GlcNAc2Fuc1 |
| N-3 | 2076.9 | 36 | SA1Hex2HexNAc2+ Man3GlcNAc2Fuc1 |
| N-4 | 2222.9 | 3.5 | SA2Hex2HexNAc2+ Man3GlcNAc2Fuc1 |
| N-5 | 2281.7 | 1 | SA1Hex2HexNAc3+ Man3GlcNAc2Fuc1 |
| N-6 | 2368.0 | 30 | SA2Hex2HexNAc2+ Man3GlcNAc2Fuc1 |
| N-7 | 2442.0 | 2 | SA1Hex3HexNAc3+ Man3GlcNAc2Fuc1 |
| N-8 | 2571.3 | 1.5 | SA2Hex2HexNAc3+ Man3GlcNAc2Fuc1 |
| N-9 | 2733.2 | 6 | SA2Hex3HexNAc3+ Man3GlcNAc2Fuc1 |
| N-10 | 3025.1 | 10 | SA3Hex3HexNAc3+ Man3GlcNAc2Fuc1 |
| N-11 | 3100.2 | 1.5 | SA2Hex4HexNAc4+ Man3GlcNAc2Fuc1 |
| N-12 | 3391.2 | 3 | SA3Hex4HexNAc4+ Man3GlcNAc2Fuc1 |
| N-13 | 3682.5 | 1.5 | SA4Hex4HexNAc4+ Man3GlcNAc2Fuc1 |
| N-14 | 3758.1 | 0.5 | SA3Hex5HexNAc5+ Man3GlcNAc2Fuc1 |
| N-15 | 4049.0 | 0.5 | SA4Hex5HexNAc5+ Man3GlcNAc2Fuc1 |
| O-linked glycan alditols | |||
| O-1 | 674.8 | 53 | SA1Hex1HexNAc1 |
| O-2 | 966.4 | 10 | SA2Hex1HexNAc1 |
| O-3 | 1040.6 | 5 | SA1Hex2HexNAc2 |
| O-4 | 1186.3 | 0.5 | SA1Hex2HexNAc2Fuc1 |
| O-5 | 1333.0 | 21 | SA2Hex2HexNAc2 |
| O-6 | 1413.8 | 1 | SA2Hex2HexNAc2(SO3)1 |
| O-7 | 1698.4 | 5 | SA2Hex3HexNAc3 |
| O-8 | 1778.7 | 2 | SA2Hex3HexNAc3(SO3)1 |
| O-9 | 1925.9 | 1 | SA2Hex3HexNAc3(SO3)1Fuc1 |
| O-10 | 2062.1 | 1 | SA2Hex4HexNAc4 |
m/z values refer to [M-H]− ions in negative-ion linear mode matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The percentages of N- and O-linked glycans have been presented separately. There are 7 times more O- than N-linked glycans on the human endothelial CD34.