Influence of NU1025 on apoptosis and colony formation in L5178Y cells treated with TZM
| In vitro treatment . | Apoptosis, % . | Colony formation, % . | |
|---|---|---|---|
| Exp 1 . | Exp 2 . | ||
| CTR | 5 ± 1 | — | — |
| TZM 15 μM | 9 ± 2 | 92 | 88 |
| TZM 30 μM | 13 ± 2 | 58 | 55 |
| NU1025 | 4 ± 1 | 100 | 100 |
| NU1025 + TZM 15 μM | 27 ± 2 | 56 | 51 |
| NU1025 + TZM 30 μM | 37 ± 3 | 17 | 15 |
| In vitro treatment . | Apoptosis, % . | Colony formation, % . | |
|---|---|---|---|
| Exp 1 . | Exp 2 . | ||
| CTR | 5 ± 1 | — | — |
| TZM 15 μM | 9 ± 2 | 92 | 88 |
| TZM 30 μM | 13 ± 2 | 58 | 55 |
| NU1025 | 4 ± 1 | 100 | 100 |
| NU1025 + TZM 15 μM | 27 ± 2 | 56 | 51 |
| NU1025 + TZM 30 μM | 37 ± 3 | 17 | 15 |
Apoptosis was assessed by flow cytometry analysis of the sub-G1 DNA content on day 1 after treatment. Data are expressed as percentage of apoptotic cells and represent the mean of 3 independent experiments, ± SE, calculated after angular transformation of the percentage values.
For colony formation, untreated or drug-treated cells were cultured in flat-bottomed, 96-well plates (10 cells/well), and after 2 weeks colonies were counted. Results are expressed as percentages of colonies formed by drug-treated cells with respect to those generated by untreated controls, and they derive from 2 independent experiments (two 96-well plates per group of treatment in each experiment).