Three donors from panel 3 were identified whose initial phenotypes did not agree with the genotypes determined by real-time polymerase chain reaction (PCR). In all 3 cases, the PCR-SSP and PCR-restriction fragment length polymorphism (RFLP) analyses confirmed the genotype determined by Taqman. Phenotyping and genotyping were subsequently repeated by using fresh samples and additional Gov antisera. Gov typing discrepancies were thereby resolved in favor of the genotypes in 2 (donors 84 and 95) of the 3 cases. Initial, results obtained from the first blood sample investigated; Repeat, results of repeat monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay and real-time PCR using fresh blood samples.