CD109-specific oligonucleotide polymerase chain reaction primers
| Fragment . | Sense primer . | Antisense primer . | Size (bp) . | Ta (°C) . | ||
|---|---|---|---|---|---|---|
| 1 | K1-80 | (−24) | K1-650 | (544) | 568 | 59 |
| 5′-GTAGCCCAGGCAGACGCC-3′ | 5′-GTGACAACCACTGTTGGATCAA-3′ | |||||
| 2 | K1-1 | (445) | K1-1120 | (1014) | 570 | 50 |
| 5′-CGCATTGTTACACTCTTCTC-3′ | 5′-TACATTTCTTGAAATACCTG-3′ | |||||
| 3 | K1-1022 | (910) | K1-REV-1 | (1747) | 838 | 50 |
| 5′-GATTCTTCAAATGGACTTT-3′ | 5′-GGCTGTGTCACAGAGATC-3′ | |||||
| 4 | K1-1400 | (1291) | GSP1 | (2165) | 875 | 55 |
| 5′-TGAATTCCCAATCCTGGAGGA-3′ | 5′-GCCACCCAAGAAGTGATAGA-3′ | |||||
| 5 | K1-M43 | (1898) | 6R4N | (2998) | 1101 | 56 |
| 5′-TTCAGGAATGTGGACTCTGG-3′ | 5′-CGGCTTCAAGGAAACATCT-3′ | |||||
| 6 | K1-3080 | (2948) | 1-5N | (3859) | 912 | 56 |
| 5′-CTGGGAGCACTTGGTTGTCA-3′ | 5′-CAGCAACATCTAAATCAAAGGC-3′ | |||||
| 7 | K1-3570 | (3462) | 7U3N | (4337) | 876 | 50 |
| 5′-ACAATTTCAGACTTCTGAGG-3′ | 5′-CACAGCCAAAGTTCCATA-3′ | |||||
| 8 | K1-3920 | (3812) | K1-4600 | (4489) | 678 | 55 |
| 5′-GACGAAGATCTATCCAAAATC-3′ | 5′-GCTAGGACCTGTTGTACACC-3′ | |||||
| Fragment . | Sense primer . | Antisense primer . | Size (bp) . | Ta (°C) . | ||
|---|---|---|---|---|---|---|
| 1 | K1-80 | (−24) | K1-650 | (544) | 568 | 59 |
| 5′-GTAGCCCAGGCAGACGCC-3′ | 5′-GTGACAACCACTGTTGGATCAA-3′ | |||||
| 2 | K1-1 | (445) | K1-1120 | (1014) | 570 | 50 |
| 5′-CGCATTGTTACACTCTTCTC-3′ | 5′-TACATTTCTTGAAATACCTG-3′ | |||||
| 3 | K1-1022 | (910) | K1-REV-1 | (1747) | 838 | 50 |
| 5′-GATTCTTCAAATGGACTTT-3′ | 5′-GGCTGTGTCACAGAGATC-3′ | |||||
| 4 | K1-1400 | (1291) | GSP1 | (2165) | 875 | 55 |
| 5′-TGAATTCCCAATCCTGGAGGA-3′ | 5′-GCCACCCAAGAAGTGATAGA-3′ | |||||
| 5 | K1-M43 | (1898) | 6R4N | (2998) | 1101 | 56 |
| 5′-TTCAGGAATGTGGACTCTGG-3′ | 5′-CGGCTTCAAGGAAACATCT-3′ | |||||
| 6 | K1-3080 | (2948) | 1-5N | (3859) | 912 | 56 |
| 5′-CTGGGAGCACTTGGTTGTCA-3′ | 5′-CAGCAACATCTAAATCAAAGGC-3′ | |||||
| 7 | K1-3570 | (3462) | 7U3N | (4337) | 876 | 50 |
| 5′-ACAATTTCAGACTTCTGAGG-3′ | 5′-CACAGCCAAAGTTCCATA-3′ | |||||
| 8 | K1-3920 | (3812) | K1-4600 | (4489) | 678 | 55 |
| 5′-GACGAAGATCTATCCAAAATC-3′ | 5′-GCTAGGACCTGTTGTACACC-3′ | |||||
Primer pairs used for the polymerase chain reaction (PCR) amplification of 8 overlapping CD109 complementary DNA (cDNA) fragments spanning the entire open reading frame (ORF) are shown. The position of the 5′ end of each oligonucleotide with respect to the published CD109 cDNA sequence10 is noted in parentheses. The CD109 ORF encompasses nucleotides 1 to 4335. The size of each PCR product (in base pairs) and the annealing temperature (Ta) used for the corresponding primer pair are listed.