Sequence analysis of clonal Ig gene rearrangements from B cells of 3 follicular lymphomas
| Case . | V gene . | Mutation frequency (%)* . | Cells with sequence variation† . | |
|---|---|---|---|---|
| Shared . | Range . | |||
| 1 | VH3-23 | 13.6 | 14.6 -16.9 | 19/30 |
| VkB3 | 6.8 | 7.3 -13.7 | 12/25 | |
| 2 | VH3-23 | 6.7 | 7.2 -9.7 | 44/71 |
| VkL2 | 3.1 | 3.1 -4.8 | 17/55 | |
| 3 | VH3-49‡ | 10.5 | 10.9 -12.6 | 11/37 |
| Vλ3r | 11.2 | 11.7 -16.1 | 13/30 | |
| Case . | V gene . | Mutation frequency (%)* . | Cells with sequence variation† . | |
|---|---|---|---|---|
| Shared . | Range . | |||
| 1 | VH3-23 | 13.6 | 14.6 -16.9 | 19/30 |
| VkB3 | 6.8 | 7.3 -13.7 | 12/25 | |
| 2 | VH3-23 | 6.7 | 7.2 -9.7 | 44/71 |
| VkL2 | 3.1 | 3.1 -4.8 | 17/55 | |
| 3 | VH3-49‡ | 10.5 | 10.9 -12.6 | 11/37 |
| Vλ3r | 11.2 | 11.7 -16.1 | 13/30 | |
All above listed cases had potentially functional rearrangements and had intraclonal sequence diversity.
All sequence variants have been deposited at the EMBL database underAJ421272-AJ421290; AJ421293-AJ421304; AJ421306-AJ421349;AJ421351-AJ421368; AJ421370-AJ421380; AJ421382-AJ421394.
Given is the frequency of shared mutations and the range of the mutation frequencies in individual V genes.
Number of variations per total number.
Two of 32 mutations detected in the direct sequence analysis were in the single-cell analysis found not to be shared by all cells of the lymphoma.