GFP⁺ cells were sorted from coculture of GFP⁺ AGM-derived CD34⁺/c-Kit⁺ with FL cells in the presence of cytokines, as indicated, at day 10. For comparison, CD34⁺/c-Kit⁺ cells and the remainder (CD34⁺/c-Kit⁻, CD34⁻/c-Kit⁺, and CD34⁻/ c-Kit⁻ cells; AGM [CD34⁺/c-Kit⁺]⁻) of primary E11.5 AGM cells were also applied to colony-formation assays. The number and type of colonies were determined at day 14. The number of each type of colony was normalized to 1 × 10⁴ cells applied to the assay. In AGM/FL coculture, 2 × 10³CD34⁺/c-Kit⁺ cells were cocultured with FL cells for 10 days, and resulted in 2.3 × 10⁶, 3.3 × 10⁶, and 1.8 × 10⁶ cells with SCF, SCF + OSM, and SCF + OSM + Dex, respectively.

AGM indicates aorta-gonad-mesonephros; FL, fetal liver; SCF, stem cell factor; OSM, oncostatin M; Dex, dexamethasone; CFU-GEMM, colony-forming unit-granulocyte, erythrocyte, macrophage, and megakaryocyte.