Thrombin-stimulated secretion of lysosomal enzymes from platelets of normal C57BL/6J and mutant mice
| . | Secretion (% total) . | |
|---|---|---|
| − thrombin . | 1.6 U thrombin . | |
| β-Glucuronidase | ||
| C57BL/6J | 5.12 ± 0.94 (6) | 46.8 ± 2.6 (5) |
| ep/ep | 4.03 ± 1.1 (6) | 56.7 ± 3.1 (6) |
| pe/pe | 4.10 ± 1.1 (6) | 15.8 ± 1.3 (6)* |
| ep/ep, pe/pe | 3.47 ± 0.68 (5) | 19.2 ± 1.1 (6)* |
| β-Galactosidase | ||
| C57BL/6J | 4.99 ± 0.77 (10) | 48.1 ± 3.0 (9) |
| ep/ep | 5.47 ± 0.96 (10) | 52.8 ± 3.3 (10) |
| pe/pe | 4.17 ± 0.37 (10) | 19.2 ± 3.6 (8)* |
| ep/ep, pe/pe | 4.21 ± 0.85 (9) | 19.6 ± 1.9 (10)* |
| . | Secretion (% total) . | |
|---|---|---|
| − thrombin . | 1.6 U thrombin . | |
| β-Glucuronidase | ||
| C57BL/6J | 5.12 ± 0.94 (6) | 46.8 ± 2.6 (5) |
| ep/ep | 4.03 ± 1.1 (6) | 56.7 ± 3.1 (6) |
| pe/pe | 4.10 ± 1.1 (6) | 15.8 ± 1.3 (6)* |
| ep/ep, pe/pe | 3.47 ± 0.68 (5) | 19.2 ± 1.1 (6)* |
| β-Galactosidase | ||
| C57BL/6J | 4.99 ± 0.77 (10) | 48.1 ± 3.0 (9) |
| ep/ep | 5.47 ± 0.96 (10) | 52.8 ± 3.3 (10) |
| pe/pe | 4.17 ± 0.37 (10) | 19.2 ± 3.6 (8)* |
| ep/ep, pe/pe | 4.21 ± 0.85 (9) | 19.6 ± 1.9 (10)* |
Platelets (1 × 108 cells/mL) were treated with thrombin for 3 min at 37°C. The reaction was terminated with Thromstop. Values represent the mean ± SEM of the individual determinations on the number of mice indicated in parentheses.
P ≤ .001.