Synergistic effect of PEG-rhuMGDF on the combination of 6GF-dependent growth of CFU-GM
| EBs6 cells . | 6GF . | Growth factors PEG-rhuMGDF . | 6GF + PEG-rhuMGDF . |
|---|---|---|---|
| mpl−/− flmpl | 37 ± 16 | 32 ± 12 | 204 ± 35* |
| mpl−/− Δ3mpl | 53 ± 13 | 16 ± 4 | 74 ± 7 |
| mpl−/− Δ34mpl | 24 ± 3 | 0 ± 0 | 28 ± 2 |
| EBs6 cells . | 6GF . | Growth factors PEG-rhuMGDF . | 6GF + PEG-rhuMGDF . |
|---|---|---|---|
| mpl−/− flmpl | 37 ± 16 | 32 ± 12 | 204 ± 35* |
| mpl−/− Δ3mpl | 53 ± 13 | 16 ± 4 | 74 ± 7 |
| mpl−/− Δ34mpl | 24 ± 3 | 0 ± 0 | 28 ± 2 |
Six-day EBs were dissociated and replated for progenitor assay in the presence of a combination of 6GF or PEG-rhuMGDF or 6GF + PEG-rhuMGDF. Data represent the mean of numbers of CFU-GM generated by 105 EBs6 cells (duplicate dishes in 3 independent experiments).
P < .01: comparison of the numbers of colonies developed with 6GF + PEG-rhuMGDF and the sum of the numbers of colonies developed with a combination of 6GF and PEG-rhuMGDF separately.