Expression of ADA cDNAs in E coli SØ3834
| ADA cDNA . | ADA activity nmol/h/mg . | Percent of wild-type . |
|---|---|---|
| Wild-type | 276 458 ± 34 428 | 100.0 |
| MutA | 3 392 ± 2 157 | 1.2 ± 0.8 |
| G360X | 270 752 ± 79 502 | 97.9 ± 28.7 |
| 360-363EPTS | 274 683 ± 40 232 | 99.3 ± 14.5 |
| 360-363EPTS + 20 | 13 627 ± 2 418 | 4.9 ± 0.9 |
| ADA cDNA . | ADA activity nmol/h/mg . | Percent of wild-type . |
|---|---|---|
| Wild-type | 276 458 ± 34 428 | 100.0 |
| MutA | 3 392 ± 2 157 | 1.2 ± 0.8 |
| G360X | 270 752 ± 79 502 | 97.9 ± 28.7 |
| 360-363EPTS | 274 683 ± 40 232 | 99.3 ± 14.5 |
| 360-363EPTS + 20 | 13 627 ± 2 418 | 4.9 ± 0.9 |
ADA activity for the vector control is 3 nmol/h/mg.6